A stress responsive alternative splicing mechanism in Citrus clementina leaves (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A stress responsive alternative splicing mechanism in Citrus clementina leaves (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.jplph.2010.11.016 (literal)

Alternative label

• Del Carratore R., Magaldi E., Podda A., Beffy P., Migheli Q., Maserti B.E. (2011)
  A stress responsive alternative splicing mechanism in Citrus clementina leaves
  in Journal of plant physiology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Del Carratore R., Magaldi E., Podda A., Beffy P., Migheli Q., Maserti B.E. (literal)

Pagina inizio

• 952 (literal)

Pagina fine

• 959 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S0176161711000125 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 168 (literal)

Rivista

• Journal of plant physiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 9 (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-IFC, Pisa, Italy; CNR-IBF, Pisa, Italy; CNR-IBF, Pisa, Italy; CNR-IFC, Pisa, Italy; Plant Protection Department, University of Sassari, Italy; CNR-IBF, Pisa, Italy (literal)

Titolo

• A stress responsive alternative splicing mechanism in Citrus clementina leaves (literal)

Abstract

• Chitinases are often considered pathogenesis-related proteins since their activity can be induced by viral infections, fungal and bacterial cell wall components, and also by more general sources of stress such as wounding, salicylic acid, ethylene, auxins and cytokinins. In the present study, comparative proteomic analysis showed the defense-related acidic chitinase II to be specifically induced in Citrus clementina leaves infested by the two-spotted spider mite Tetranychus urticae or treated with MeJA. In parallel, changes in the mRNA profiles of two partially homologous chitinase forms were shown by RT-PCR. In particular, the appearance of an additional cDNA chitinase fragment in T. urticae-infested and MeJA-treated leaves was observed. This finding may indicate a specific regulatory mechanism of chitinase expression. We report evidence for alternative splicing in T. urticae-infested C. clementina, where a premature stop codon after the first 135 amino acids was introduced. We observed inducible chitinase activity after MeJA treatment, indicative of a rapid plant response to infestation. This work provides the first evidence of chitinase alternative splicing in C. clementina. In addition, the presence of the dual-band pattern for chitinase cDNA by RT-PCR may represent a suitable predictive marker for early diagnosis of plant biotic stress (literal)

Prodotto di

• Biofisica e biologia molecolare nello studio dei processi ambientali (TA.P04.030.001) (Modulo)
• Istituto di fisiologia clinica (IFC) (Istituto)
• Istituto di biofisica (IBF) (Istituto)

Autore CNR

• Alessandra PODDA (Persona)
• BIANCAELENA MASERTI (Persona)
• MARIA RENATA DEL CARRATORE (Persona)

Insieme di parole chiave

• Keywords of "A stress responsive alternative splicing mechanism in Citrus clementina leaves" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Istituto di fisiologia clinica (IFC) (Istituto)
• Biofisica e biologia molecolare nello studio dei processi ambientali (TA.P04.030.001) (Modulo)

Autore CNR di

• BIANCAELENA MASERTI (Persona)
• Alessandra PODDA (Persona)
• MARIA RENATA DEL CARRATORE (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of plant physiology (Rivista)

Insieme di parole chiave di

• Keywords of "A stress responsive alternative splicing mechanism in Citrus clementina leaves" (Insieme di parole chiave)