Microalgal cryopreservation using Dimethyl Sulfoxide (Me2SO) coupled with two freezing protocols: influence on the fatty acid profile (Articolo in rivista)

Type
Label
  • Microalgal cryopreservation using Dimethyl Sulfoxide (Me2SO) coupled with two freezing protocols: influence on the fatty acid profile (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Guermazi W., Sellami-kammoun A., Elloumi J., Drira Z., Aleya L., MARANGONI R., Ayadi H., Maalej S. (2010)
    Microalgal cryopreservation using Dimethyl Sulfoxide (Me2SO) coupled with two freezing protocols: influence on the fatty acid profile
    in Journal of thermal biology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Guermazi W., Sellami-kammoun A., Elloumi J., Drira Z., Aleya L., MARANGONI R., Ayadi H., Maalej S. (literal)
Pagina inizio
  • 175 (literal)
Pagina fine
  • 181 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 35 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Microalgal cryopreservation using Dimethyl Sulfoxide (Me2SO) coupled with two freezing protocols: influence on the fatty acid profile (literal)
Abstract
  • Procedures for determining the optimal pre-freezing protocol for cryo-preservation of microalgae are discussed. Three algal species were used (Chlorella vulgaris, Isochrysis galbana and Dunaliella salina) and cryo-stored using two different methods: the slow cooling and the fast freezing. In the slow cooling, each algae batch was treated with or without cryo-protectant (dimethyl sulfoxide: Me2SO 5% v/v). After 20 min at 4 degrees C, the midi-straws were filled and cooled slowly (1.5 degrees C min(-1)) to -140 degrees C, by a programmable freezer (Digitcool-IMV), before putting them directly into liquid nitrogen. Fast freezing was performed with 10% or 15% Me2SO prior to plunging into liquid nitrogen. The three algal species followed the same re-growth pattern as that of the controls. The post-thawed viability with Me2SO was good for all the selected algae (C. vulgaris > 95%, I. galbana and D. sauna > 70% of the control), applying the slow cooling. The post-thawed viability without Me2SO was 60% for I. galbana, 52% for D. salina and 33% for C. vulgaris. Fast freezing was not suitable for cryo-storage of I. galbana but gave good post-thawing viability for D. salina (70%). The decrease in fatty acid content of the cryo-stored algae was influenced by the temperature. The rapid decrease in temperature induced by fast freezing can explain the low level of fatty acid content of the three cryo-stored algae. Fatty acid profiles show that the nutritional values of the three cryo-stored micro-algae were not significantly affected especially when treated with slow cooling protocols. (literal)
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