Development of real-time PCR systems based on SYBR green I and TaqMan technologies for specific quantitative detection of Phoma tracheifila in infected Citrus (Articolo in rivista)

Type
Label
  • Development of real-time PCR systems based on SYBR green I and TaqMan technologies for specific quantitative detection of Phoma tracheifila in infected Citrus (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s10658-007-9222-9 (literal)
Alternative label
  • Demontis M.A., Cacciola S.O., Orrù M., Balmas V., Chessa V., Maserti B.E., Mascia L., Raudino F., Magnano di San Lio G., Migheli Q. (2008)
    Development of real-time PCR systems based on SYBR green I and TaqMan technologies for specific quantitative detection of Phoma tracheifila in infected Citrus
    in European journal of plant pathology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Demontis M.A., Cacciola S.O., Orrù M., Balmas V., Chessa V., Maserti B.E., Mascia L., Raudino F., Magnano di San Lio G., Migheli Q. (literal)
Pagina inizio
  • 339 (literal)
Pagina fine
  • 351 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.springerlink.com/content/9u6t258633g59v64/?MUD=MP (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 120 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 4 (literal)
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Unità di Ricerca, Istituto Nazionale Biostrutture e Biosistemi, Università di Sassari, Via E. De Nicola 9, 07100 Sassari, Italy b Dipartimento di Protezione Delle Piante, Università di Sassari, Via E. De Nicola 9, 07100 Sassari, Italy c Dipartimento S.En.Fi.Mi.Zo., Università di Palermo, Viale delle Scienze 2, 90128 Palermo, Italy d Consiglio Nazionale Delle Ricerche, Istituto di BioFisica, Area Della Ricerca, Via Moruzzi 1, 56124 Pisa, Italy e Dipartimento di Gestione dei Sistemi Agrari e Forestali, Università Mediterranea di Reggio Calabria, Località Feo di Vito, 89060 Reggio Calabria, Italy (literal)
Titolo
  • Development of real-time PCR systems based on SYBR green I and TaqMan technologies for specific quantitative detection of Phoma tracheifila in infected Citrus (literal)
Abstract
  • Mal secco disease of citrus caused by Phoma tracheiphila is a devastating disease in the Mediterranean basin. Susceptible citrus species include lemon, citron, lime and others. Trees attacked by the fungus show characteristic symptoms; the smallest twigs die first, followed by the larger branches. Eventually, the whole tree is killed. The symptoms are clear in the orchards but by the time they are visible the disease is already well established. The need for a sensitive, reliable and rapid diagnostic method for the early identification of the fungus in trees and fruit exists. We have developed a PCR-based method for the identification of P. tracheiphila from plant tissues including fruit. Any such method must take into account the genetic variability in the pathogen population. Molecular methods were used to compare different isolates of P. tracheiphila. This study found no significant differences between different isolates from different citrus species from different parts of Israel. (literal)
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