beta1 subunit modulates the Nav1.4 sodium channel by changing the surface charge. (Articolo in rivista)

Type
Label
  • beta1 subunit modulates the Nav1.4 sodium channel by changing the surface charge. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ferrera L., Moran O. (2006)
    beta1 subunit modulates the Nav1.4 sodium channel by changing the surface charge.
    in Experimental brain research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ferrera L., Moran O. (literal)
Pagina inizio
  • 139 (literal)
Pagina fine
  • 150 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 172 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biofisica, CNR, Genova (literal)
Titolo
  • beta1 subunit modulates the Nav1.4 sodium channel by changing the surface charge. (literal)
Abstract
  • Voltage-gated sodium channels, comprised of a pore-forming alpha-subunit and additional regulatory (beta) subunits, play a critical role in regulation of neuronal excitability. Mechanisms of regulation of beta-subunits remain elusive. We have tested the functional effects of beta1 sodium channel subunit on surface charges as a mechanism for channel modulation. HEK-293 cell lines permanently transfected with the sole rat skeletal muscle sodium channel alpha-subunit (Nav1.4), or co-expressing the sodium channel alpha-subunit and beta1-subunit were studied with the whole-cell mode of the patch-clamp technique. At physiological extracellular Ca(2+) concentration (2 mM), expression of beta1-subunit did not produce any significant effect on the voltage-dependent properties of sodium currents. However, a shift of half-activation potentials of sodium channel by changing the extracellular Ca(2+) was potentiated when beta1 was co-expressed with alpha-subunit. In contrast, the expression of beta1-subunit did not affect the Ca(2+) binding to the open or to the closed sodium channel pore, difference of the effect provoked by extracellular Ca(2+) could therefore be attributed to an increased in negative surface charge determined by the presence of beta1-subunit. These data are in agreement with the hypothesis of a modulation of the sodium current by the expression of the highly sialylated beta1-subunit, which would alter the channel gating by increasing the density of surface negative charges in the vicinity of the sodium channel voltage sensing machinery. (literal)
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