Biosynthesis of Cd-bound Phytochelatins (PCs) by Phaeodactylum tricornutum and their Speciation via Size-Exclusion Chromatography (SEC) and Ion-Pairing Chromatography (IPC) coupled to ICP-MS. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Biosynthesis of Cd-bound Phytochelatins (PCs) by Phaeodactylum tricornutum and their Speciation via Size-Exclusion Chromatography (SEC) and Ion-Pairing Chromatography (IPC) coupled to ICP-MS. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00216-005-3385-z (literal)

Alternative label

• Loreti V.; Toncelli D.; Morelli E.; Scarano G.; Bettmer J. (2005)
  Biosynthesis of Cd-bound Phytochelatins (PCs) by Phaeodactylum tricornutum and their Speciation via Size-Exclusion Chromatography (SEC) and Ion-Pairing Chromatography (IPC) coupled to ICP-MS.
  in Analytical & bioanalytical chemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Loreti V.; Toncelli D.; Morelli E.; Scarano G.; Bettmer J. (literal)

Pagina inizio

• 398 (literal)

Pagina fine

• 403 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 383 (literal)

Rivista

• Analytical & bioanalytical chemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• V. Loreti, D. Toncelli, J. Bettmer - Institute of Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg-University of Mainz, Duesbergweg 10-14, 55099 Mainz, Germany; E. Morelli, G. Scarano - Istituto di Biofisica, Consiglio Nazionale delle Ricerche (CNR), Area della ricerca di Pisa, via Moruzzi 1, 56124 Pisa, Italy (literal)

Titolo

• Biosynthesis of Cd-bound Phytochelatins (PCs) by Phaeodactylum tricornutum and their Speciation via Size-Exclusion Chromatography (SEC) and Ion-Pairing Chromatography (IPC) coupled to ICP-MS. (literal)

Abstract

• Cd-bound phytochelatins (Cd-PCs) have been synthesised by incubation of Phaeodactylum tricornutum cell cultures with Cd and purified by size-exclusion chromatography-UV-Vis. These complexes, which were identified in previous work, have now been used as model substances to develop and optimise ion-pair chromatography (IPC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS) for analysis of Cd-PCs. Subsequent analysis of samples taken from Silene vulgaris plants cultivated under heavy metal stress conditions revealed Cd signals but no Cd-PC signals. By use of isotopically enriched (116)Cd-PCs the sample preparation steps were verified to determine the stability of the analytes. We observed species transformation between Cd-PCs and other unidentified Cd complexes. Consequently, the kinetic and thermodynamic lability of Cd-PCs are decisive factors in their detection. (literal)

Prodotto di

• Meccanismi di risposta cellulare a contaminanti ambientali (TA.P05.015.002) (Modulo)
• Istituto di biofisica (IBF) (Istituto)

Autore CNR

• ELISABETTA MORELLI (Unità di personale interno)
• GIOACCHINO SCARANO (Persona)

Insieme di parole chiave

• Parole chiave di "Biosynthesis of Cd-bound Phytochelatins (PCs) by Phaeodactylum tricornutum and their Speciation via Size-Exclusion Chromatography (SEC) and Ion-Pairing Chromatography (IPC) coupled to ICP-MS." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Meccanismi di risposta cellulare a contaminanti ambientali (TA.P05.015.002) (Modulo)

Autore CNR di

• ELISABETTA MORELLI (Unità di personale interno)
• GIOACCHINO SCARANO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Analytical & bioanalytical chemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "Biosynthesis of Cd-bound Phytochelatins (PCs) by Phaeodactylum tricornutum and their Speciation via Size-Exclusion Chromatography (SEC) and Ion-Pairing Chromatography (IPC) coupled to ICP-MS." (Insieme di parole chiave)