Two-photon analysis of lead accumulation in rat cerebellar granule cells (Articolo in rivista)

Type
Label
  • Two-photon analysis of lead accumulation in rat cerebellar granule cells (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s11064-005-5980-y (literal)
Alternative label
  • Esposito A.; Robello M.; Pellistri F; Marchetti C. (2005)
    Two-photon analysis of lead accumulation in rat cerebellar granule cells
    in Neurochemical research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Esposito A.; Robello M.; Pellistri F; Marchetti C. (literal)
Pagina inizio
  • 949 (literal)
Pagina fine
  • 954 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.springerlink.com/content/p506641131864833/?MUD=MP (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 30 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 8 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Genoa, Dipartimento Fis, INFM, I-16146 Genoa, Italy European Neurosci Inst, Cell Biophys Grp, D-37073 Gottingen, Germany CNR, Ist Biofis, I-16149 Genoa, Italy (literal)
Titolo
  • Two-photon analysis of lead accumulation in rat cerebellar granule cells (literal)
Abstract
  • Lead (Pb2+) is a common pollutant and potent central neurotoxin. We have studied its pathways of permeation by two-photon fluorescence microscopy in rat cerebellar granule neurons loaded with the fluorescent dye indo-1. Pb2+ binds indo-1 with high affinity acting as a quencher. Its permeation through the neuronal membrane was indicated by a decrease of the fluorescence emission, which occurred even in resting condition. In the presence of 20 microM Pb2+, uptake reached a plateau level (approximately 45% of initial fluorescence) in 4 min and was partially antagonized by 25 microM lanthanum. Subsequent addition of a membrane permeant ionophore caused a further (>70%) quenching of the dye, suggesting that previous saturation was due to inactivation of the transport system. Intracellular Pb2+ concentrations were evaluated from the fluorescence intensity and this estimate indicated that the concentration of free Pb2+ sufficient to inactivate the transport system is close to 50 pM. (literal)
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