Isolation and Structural Characterization of two Water-Borne Pheromones from Euplotes crassus, a Ciliate Commonly Known to Carry Membrane-Bound Pheromones (Articolo in rivista)

Type
Label
  • Isolation and Structural Characterization of two Water-Borne Pheromones from Euplotes crassus, a Ciliate Commonly Known to Carry Membrane-Bound Pheromones (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Alimenti C, Vallesi A, Federici S, Di Giuseppe G, Dini F, Carratore V, Luporini PA (2011)
    Isolation and Structural Characterization of two Water-Borne Pheromones from Euplotes crassus, a Ciliate Commonly Known to Carry Membrane-Bound Pheromones
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Alimenti C, Vallesi A, Federici S, Di Giuseppe G, Dini F, Carratore V, Luporini PA (literal)
Pagina inizio
  • 234 (literal)
Pagina fine
  • 241 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 58 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Isolation and Structural Characterization of two Water-Borne Pheromones from Euplotes crassus, a Ciliate Commonly Known to Carry Membrane-Bound Pheromones (literal)
Abstract
  • Ciliates comprise species synthesizing water-diffusible mating type factors or pheromones and species synthesizing insoluble, cell membrane-bound pheromones. Euplotes crassus has traditionally been placed in the latter group. In contrast with this notion, we found that E. crassus is a constitutive pheromone-secreting ciliate, like other Euplotes species. From cell-free filtrate preparations of the E. crassus strain L-2D, we isolated two distinct pheromones, designated as Ec-± and Ec-1, and determined their complete amino acid sequences by combined chemical and genetic approaches. The Ec-± pheromone sequence extends for 56 amino acid residues with six cysteines and shows a molecular mass of 6,183 Da, while the Ec-1 pheromone sequence extends for 45 amino acid residues with 10 cysteines and shows a molecular mass of 4,840 Da. Marked structural differences distinguish the full-length Ec-± and Ec-1 coding sequences, which have been cloned and characterized from the transcriptionally active macronuclear genome. They were taken as clear indication that the Ec-± and Ec-1 pheromones are specified by genes that are not allelic, but likely derived from a duplicated genetic locus of the transcriptionally silent micronuclear genome (literal)
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