Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins. (Articolo in rivista)

Type
Label
  • Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins. (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Vergara A, Vitagliano L, Verde C, di Prisco G, Mazzarella L. (2008)
    Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins.
    in Methods in enzymology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Vergara A, Vitagliano L, Verde C, di Prisco G, Mazzarella L. (literal)
Pagina inizio
  • 425 (literal)
Pagina fine
  • 444 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 436 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins. (literal)
Abstract
  • Hemoglobins (Hbs) are important proteins devoted to oxygen transport. Hbs carry out their function by keeping the iron atom, which binds the oxygen molecule, in its reduced Fe(II) state. Nonetheless, it is well known that Hbs frequently undergo, even under physiological conditions, spontaneous oxidation. Although these processes have been widely investigated, their role and impact in different biological contexts are still highly debated. In vertebrate Hbs, assembled in ±2²2 tetramers, it has traditionally been assumed that oxidized forms endowed with nativelike structures are either aquo-met or hydroxy-met states, depending on the pH of the medium. This view has been questioned by several independent investigations. In the past, indirect evidence of the existence of alternative nativelike oxidized forms was obtained from spectroscopic analyses. Indeed, it was suggested that, in tetrameric Hbs, bis-histidyl hemichrome states could be compatible with folded structures. Recent studies performed by complementing spectroscopic and crystallographic methodologies have provided a detailed picture of hemichrome structure and formation in these proteins. Here we review the methodological approaches adopted to achieve these results, the main structural features of these states, and the current hypotheses on their possible functional implications. (literal)
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