http://www.cnr.it/ontology/cnr/individuo/prodotto/ID88392

No production in tomato plants under biotic stress. (Contributo in atti di convegno)

Type

Contributo in atti di convegno (Classe)
Prodotto della ricerca (Classe)

Label

No production in tomato plants under biotic stress. (Contributo in atti di convegno) (literal)

Anno

2003-01-01T00:00:00+01:00 (literal)

Alternative label

Melillo M.T., Leone A., Veronico P., Bleve-Zacheo T. (2003)
No production in tomato plants under biotic stress.
in 30th National Congress of the Italian Society of Histochemistry, Rapallo (Genova), 25-28 Maggio 2003
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Melillo M.T., Leone A., Veronico P., Bleve-Zacheo T. (literal)

Pagina inizio

28 (literal)

Pagina fine

28 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

47 (literal)

Rivista

European journal of histochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

European Journal of Histochemistry, 47, Suppl.1, 28. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

1 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

1 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

A real-time reverse transcription (RT) PCR assay was developed to assess presence of Grapevine fanleaf virus (GFLV) in single specimens of its vector Xiphinema index. The test used fluorescent DNA-based probes and a 660 bp cloned fragment of the GFLV coat protein (CP) gene. The X. index population proceeded from a grapevine orchard at Palagiano, Italy. A diluted series of in vitro transcripts from the fragment served as standard to quantify the virus load in nematodes. The test allowed recognition of individual specimens with adsorbed GFLV particles. Positive specimens were distinguished from controls or negative samples by the fluorescence emissions observed during amplification. Fluorescence from positive nematodes rised at cycles 3032, and corresponded to a minimum amount of template RNA of about 800 fg. Considering that GFLV particles have a single CP copy, the minimum amount of virus detectable was approx. 1.06 10 6 particles per nematode. The CP fragments amplified and sequenced from other specimens of the Palagiano population showed a low rate of nucleotide variability, as only 0.2% of positions displayed a nucleotide substitution. They did not involve, however, the region used for detection. This region appeared highly conserved in GFLV, as other strains amplified and sequenced from dry grapevine leaves proceeding from Malta were similar to the italian strain used. The assay allows reliable quantitative detection of GFLV from single specimens of X. index, as well as the monitoring of the nematodes capability to acquire, retain and/or transmit the virus. (literal)

Note