Application of a Site-specific DNA Excision Strategy for Marker Gene Removal during Gene Transfer in Vitis spp. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Application of a Site-specific DNA Excision Strategy for Marker Gene Removal during Gene Transfer in Vitis spp. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• MartinelliI, L., Dalla Costa, L., Vaccari, I., Poletti, V., Gribaudo,I., Gambino, G., Guzzo, F., Saldarelli, P., Minafra, A., Turturo,C. (2009)
  Application of a Site-specific DNA Excision Strategy for Marker Gene Removal during Gene Transfer in Vitis spp.
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• MartinelliI, L., Dalla Costa, L., Vaccari, I., Poletti, V., Gribaudo,I., Gambino, G., Guzzo, F., Saldarelli, P., Minafra, A., Turturo,C. (literal)

Pagina inizio

• 399 (literal)

Pagina fine

• 403 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 827 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Proc.9th International Conference on Grape Genetics and Breeding, Udine 2006. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento Scientifico e Tecnologico, Università di Verona, Italy IASMA Research Center, Genetics and Molecular Biology Department, Via E. Mach, 1 – 38010 San Michele all’Adige (TN) (literal)

Titolo

• Application of a Site-specific DNA Excision Strategy for Marker Gene Removal during Gene Transfer in Vitis spp. (literal)

Abstract

• Site-specific DNA excision strategy for the marker gene removal was exploited for gene transfer into grape. Nicotiana benthamiana leaf sections and embryogenic calli of V. vinifera (cvs. Chardonnay and Brachetto) were co-cultured with Agrobacterium tumefaciens carrying the chemically-inducible pX6 vector with the Green Fluorescent Protein gene (GFP). This vector provides a reliable system for the marker gene (NPTII) excision, based on Cre/loxP and regulated by 17-b-estradiol. Putatively transgenic cultures were selected on kanamycin, and plantlets were regenerated. Preliminary molecular assays showed the transfer of the GFP gene into the plant genome. After inductions of the cultures on different concentration and exposition time on 17-b-estradiol, observations at the fluorescence stereomicroscope showed the expected GFP gene expression. Besides, to exploit the effectiveness of this strategy for achieving Pathogen Derived Resistance, the GFP gene was replaced with a sequence of the GVA virus coat protein gene in sense and antisense orientation for transcription of an hairpin RNA (pX6-pKcpGVA). Gene transfer experiments on tobacco and grapes produced plantlets containing the foreign genes. (literal)

Prodotto di

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Autore CNR

• PASQUALE SALDARELLI (Persona)
• IVANA GRIBAUDO (Persona)
• ANGELANTONIO MINAFRA (Persona)
• GIORGIO GAMBINO (Persona)

Insieme di parole chiave

• Keywords of "Application of a Site-specific DNA Excision Strategy for Marker Gene Removal during Gene Transfer in Vitis spp." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ANGELANTONIO MINAFRA (Persona)
• PASQUALE SALDARELLI (Persona)
• IVANA GRIBAUDO (Persona)
• GIORGIO GAMBINO (Persona)

Prodotto

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Insieme di parole chiave di

• Keywords of "Application of a Site-specific DNA Excision Strategy for Marker Gene Removal during Gene Transfer in Vitis spp." (Insieme di parole chiave)