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Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow (Articolo in rivista)

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Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow (Articolo in rivista) (literal)

Anno

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Alternative label

A. Polini, E. Mele, A. G. Sciancalepore, S. Girardo, A. Biasco, A. Camposeo, R. Cingolani, D. A. Weitz, D. Pisignano (2010)
Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow
in Biomicrofluidics
(literal)

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A. Polini, E. Mele, A. G. Sciancalepore, S. Girardo, A. Biasco, A. Camposeo, R. Cingolani, D. A. Weitz, D. Pisignano (literal)

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Rivista

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Issue: 3 Article Number: 036502 Acknowledgement: The authors are grateful to the support of the Apulia Regional Strategic Project PS_144, and of the Italian Ministry of University and Research through the FIRB Project Nos. RBIN045MNB and RBLA03ER38. (literal)

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1. NNL, National Nanotechnology Laboratory of CNR-Istituto Nanoscienze, Università del Salento, I-73100 Lecce, Italy 2. Scuola Superiore ISUFI, Università del Salento, I-73100 Lecce, Italy 3. Istituto Italiano di Tecnologia (I.I.T.), Center for Biomolecular Nanotechnologies, I-73100 Arnesano (LE), Italy 4. Istituto Italiano di Tecnologia (I.I.T.), I-16163 Genova, Italy 5. Department of Physics and School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA (literal)

Titolo

Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow (literal)

Abstract

Producing polymeric or hybrid microfluidic devices operating at high temperatures with reduced or no water evaporation is a challenge for many on-chip applications including polymerase chain reaction (PCR). We study sample evaporation in polymeric and hybrid devices, realized by glass microchannels for avoiding water diffusion toward the elastomer used for chip fabrication. The method dramatically reduces water evaporation in PCR devices that are found to exhibit optimal stability and effective operation under oscillating-flow. This approach maintains the flexibility, ease of fabrication, and low cost of disposable chips, and can be extended to other high-temperature microfluidic biochemical reactors. (C) 2010 American Institute of Physics. (literal)

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