http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56918

Somatic embryogenesis efficiently eliminates viroid infections from grapevines (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Somatic embryogenesis efficiently eliminates viroid infections from grapevines (Articolo in rivista) (literal)

Anno

2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1007/s10658-011-9770-x (literal)

Alternative label

Gambino G., Navarro B. , Vallania R., Gribaudo I., Di Serio F. (2011)
Somatic embryogenesis efficiently eliminates viroid infections from grapevines
in European journal of plant pathology
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Gambino G., Navarro B. , Vallania R., Gribaudo I., Di Serio F. (literal)

Pagina inizio

511 (literal)

Pagina fine

519 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://link.springer.com/article/10.1007%2Fs10658-011-9770-x (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

130 (literal)

Rivista

European journal of plant pathology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

IMPACT FACTOR 1.575 (literal)

Note

Scopu (literal)
ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Istituto di Virologia Vegetale del CNR (IVV) (literal)

Titolo

Somatic embryogenesis efficiently eliminates viroid infections from grapevines (literal)

Abstract

Indirect somatic embryogenesis is effective at eliminating the most important viruses affecting grapevines. Accordingly, this technique was tested as a method for eradicating two widespread viroids, Grapevine yellow speckle viroid 1 (GYSVd-1) and Hop stunt viroid (HSVd), from four grapevine cultivars. Both viroids were detected by RT-PCR in grapevine floral organs used for initiating embryogenic cultures, as well as in undifferentiated cells of embryogenic and non-embryogenic calli from anthers and ovaries. In contrast, somatic embryos differentiated from these infected calli were viroid-free, and viroids were not detected in embryo-derived plantlets even three years after their transfer to greenhouse conditions. A wider spatial distribution of HSVd than GYSVd-1 within proliferating calli was revealed by in situ hybridization, whereas no hybridization signal was detected in the somatic embryos. In addition, GYSVd-1 and HSVd were localised in the nucleus of infected cells, conclusively showing the nuclear accumulation of representative members of Apscaviroid and Hostuviroid genera, which was only an assumption, so far. Somatic embryogenesis was compared to in vitro thermotherapy, a technique routinely used for virus eradication. After thermotherapy, HSVd and GYSVd-1 were detected in all in vitro plantlets of the cultivar Roussan, and in all lines analysed after 3 years of culture in greenhouse. The high efficiency with which somatic embryogenesis may eliminate viroids and viruses from several infected grapevine cultivars, should allow to obtain virus- and viroid-free material, which would be useful not only for sanitary selection but also for basic research on plant-virus and plant-viroid interactions in grapevine. (literal)

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