Citrus tristeza virus infection induces the accumulation of viral small RNAs (21 24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Citrus tristeza virus infection induces the accumulation of viral small RNAs (21 24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s11103-011-9754-4 (literal)

Alternative label

• Ruiz-Ruiz S., Navarro B., Gisel A., Pena L., Navarro L., Moreno P., Di Serio F., Flores R. (2011)
  Citrus tristeza virus infection induces the accumulation of viral small RNAs (21 24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile
  in Plant molecular biology; SPRINGER, DORDRECHT (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Ruiz-Ruiz S., Navarro B., Gisel A., Pena L., Navarro L., Moreno P., Di Serio F., Flores R. (literal)

Pagina inizio

• 607 (literal)

Pagina fine

• 619 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• The first two authors have contributed equally to this work PMID: 21327514 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.springerlink.com/content/827043361r188304/ (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 75 (literal)

Rivista

• Plant molecular biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• IMPACT FACTOR 3.978 (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Instituto de Biologia Molecular y Celular de Plantas, Universidad Polite´cnica de Valencia-Consejo Superior de Investigaciones Cientificas, Avenida de los Naranjos, 46022-Valencia, Spain 2. CNR, Istituto di Virologia Vegetale, I-70126 Bari, Italy 3. CNR, Istituto Tecnologie Biomediche, I-70126 Bari, Italy 4. Instituto Valenciano de Investigaciones Agrarias, 46113-Moncada, Valencia, Spain (literal)

Titolo

• Citrus tristeza virus infection induces the accumulation of viral small RNAs (21 24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile (literal)

Abstract

• To get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (Solexa-Illumina) the small RNAs (sRNAs) in three virus-host combinations. Our data show that CTV sRNAs: (i) represent more than 50% of the total sRNAs in Mexican lime and sweet orange (where CTV reaches relatively high titers), but only 3.5% in sour orange (where the CTV titer is significantly lower), (ii) are predominantly of 21–22-nt, with a biased distribution of their 50 nucleotide and with those of (?) polarity accumulating in a moderate excess, and (iii) derive from essentially all the CTV genome (ca. 20 kb), as revealed by its complete reconstruction from viral sRNA contigs, but adopt an asymmetric distribution with a prominent hotspot covering approximately the 30-terminal 2,500 nt. These results suggest that the citrus homologues of Dicer-like (DCL) 4 and 2 most likely mediate the genesis of the 21 and 22 nt CTV sRNAs, respectively, and show that both ribonucleases act not only on the genomic RNA but also on the 30 co-terminal subgenomic RNAs and, particularly, on their double-stranded forms. The plant sRNA profile, very similar and dominated by the 24-nt sRNAs in the three mock-inoculated controls, was minimally affected by CTV infection in sour orange, but exhibited a significant reduction of the 24-nt sRNAs in Mexican lime and sweet orange. We have also identified novel citrus miRNAs and determined how CTV influences their accumulation (literal)

Editore

• SPRINGER (Editore)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• ANDREAS GISEL (Persona)
• BEATRIZ NAVARRO RAMIREZ (Persona)
• FRANCESCO DI SERIO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Citrus tristeza virus infection induces the accumulation of viral small RNAs (21 24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• BEATRIZ NAVARRO RAMIREZ (Persona)
• ANDREAS GISEL (Persona)
• FRANCESCO DI SERIO (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant molecular biology (Rivista)

Editore di

• SPRINGER (Editore)

Insieme di parole chiave di

• Parole chiave di "Citrus tristeza virus infection induces the accumulation of viral small RNAs (21 24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile" (Insieme di parole chiave)