http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56852

Deep Sequencing of the Small RNAs Derived from two Symptomatic Variants of a Chloroplastic Viroid: Implications for their Genesis and for Viroid Pathogenesis (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Deep Sequencing of the Small RNAs Derived from two Symptomatic Variants of a Chloroplastic Viroid: Implications for their Genesis and for Viroid Pathogenesis (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1371/journal.pone.0007539 (literal)

Alternative label

Di Serio F.(1); Gisel A.(2); Navarro B.(1), Delgado S.(3); Martínez de Alba Á.E. (3); Donvito G. (4); Flores R. (3) (2009)
Deep Sequencing of the Small RNAs Derived from two Symptomatic Variants of a Chloroplastic Viroid: Implications for their Genesis and for Viroid Pathogenesis
in PloS one; Public Library of Science, San Francisco (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Di Serio F.(1); Gisel A.(2); Navarro B.(1), Delgado S.(3); Martínez de Alba Á.E. (3); Donvito G. (4); Flores R. (3) (literal)

Pagina inizio

e7539 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0007539 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

4 (literal)

Rivista

PloS one (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

doi: 10.1371/journal.pone.0007539 IMPACT FACTOR 2008: 5.00 (literal)

Note

ISI Web of Science (WOS) (literal)
Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

(1) Istituto di Virologia vegetale del CNR, 70126 Bari, Italy (2) Istituto di Tecnologie Biomediche del CNR, 70126 Bari, Italy. (3) Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), 46022 Valencia, Spain. (4) Istituto Nazionale di Fisica Nucleare, 70126 Bari, Italy. (literal)

Titolo

Deep Sequencing of the Small RNAs Derived from two Symptomatic Variants of a Chloroplastic Viroid: Implications for their Genesis and for Viroid Pathogenesis (literal)

Abstract

Northern-blot hybridization and low-scale sequencing have revealed that plants infected by viroids, non-protein-coding RNA replicons, accumulate 21-24 nt viroid-derived small RNAs (vd-sRNAs) similar to the small interfering RNAs, the hallmarks of RNA silencing. These results strongly support that viroids are elicitors and targets of the RNA silencing machinery of their hosts. Low-scale sequencing, however, retrieves partial datasets and may lead to biased interpretations. To overcome this restraint we have examined by deep sequencing (Solexa-Illumina) and computational approaches the vd-sRNAs accumulating in GF-305 peach seedlings infected by two molecular variants of Peach latent mosaic viroid (PLMVd) inciting peach calico (albinism) and peach mosaic. Our results show in both samples multiple PLMVd-sRNAs, with prevalent 21-nt (+) and (-) RNAs presenting a biased distribution of their 5 nucleotide, and adopting a hotspot profile along the genomic (+) and (-) RNAs. Dicer-like 4 and 2 (DCL4 and DCL2, respectively), which act hierarchically in antiviral defense, most likely also mediate the genesis of the 21- and 22-nt PLMVd-sRNAs. More specifically, because PLMVd replicates in plastids wherein RNA silencing has not been reported, DCL4 and DCL2 should dice the PLMVd genomic RNAs during their cytoplasmic movement or the PLMVd-dsRNAs generated by a cytoplasmic RNA-dependent RNA polymerase (RDR), like RDR6, acting in concert with DCL4 processing. Furthermore, given that vd-sRNAs derived from the 12-14-nt insertion containing the pathogenicity determinant of peach calico are underrepresented, it is unlikely that symptoms may result from the accidental targeting of host mRNAs by vd-sRNAs from this determinant guiding the RNA silencing machinery. (literal)

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