Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium-mediated transformation. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium-mediated transformation. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s11032-009-9293-8 (literal)

Alternative label

• Giorgio Gambino; Walter Chitarra; Fatemeh Maghuly; Margit Laimer; Paolo Boccacci; Daniela Torello Marinoni; Ivana Gribaudo (2009)
  Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium-mediated transformation.
  in Molecular breeding
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Giorgio Gambino; Walter Chitarra; Fatemeh Maghuly; Margit Laimer; Paolo Boccacci; Daniela Torello Marinoni; Ivana Gribaudo (literal)

Pagina inizio

• 305 (literal)

Pagina fine

• 320 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 24 (literal)

Rivista

• Molecular breeding (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• IMPACT FACTOR 2008: 2.008 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Plant Virology Institute (CNR), Grugliasco Unit, Via L. da Vinci 44, 10095 Grugliasco, TO, Italy. Department of Arboriculture and Pomology, University of Torino. Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy. Plant Biotechnology Unit, Institute of Applied Microbiology BOKU, Nussdorfer Lände 11, A- 1190 Wien, Austria. (literal)

Titolo

• Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium-mediated transformation. (literal)

Abstract

• T-DNA integration patterns in 49 transgenic grapevines produced via Agrobacterium-mediated transformation were analyzed. Inverse PCR (iPCR) was performed to identify T-DNA/plant junctions. Sequence comparison revealed several deletions in the T-DNA right border (RB) and left border (LB), and filler DNA and duplications or deletions of grapevine DNA at the T-DNA insertion loci. In 20 T-DNA/grapevine genome junctions microsimilarities were found associated with the joining points and in all grapevine lines microsimilarities were present near the breaking points along the 30 bases of T-DNA adjacent to the two borders. Analysis of target site preferences of T-DNA insertions indicated a non-random distribution of the T-DNA, with a bias toward the intron regions of the grapevine genes. Compositional analysis of grapevine DNA around the T-DNA insertion sites revealed an inverse relationship between the CG and AT-skews and AT rich sequences present at 300-500 bp upstream the insertion points, near the RB of the T-DNA. PCR assays showed that vector backbone sequences were integrated in 28.6 % of the transgenic plants analyzed and multiple T-DNAs frequently integrated at the same position in the plant genome, resulting in the formation of tandem and inverted repeats (literal)

Prodotto di

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Autore CNR

• PAOLO BOCCACCI (Persona)
• GIORGIO GAMBINO (Persona)
• IVANA GRIBAUDO (Persona)

Insieme di parole chiave

• Parole chiave di "Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium-mediated transformation." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• IVANA GRIBAUDO (Persona)
• GIORGIO GAMBINO (Persona)
• PAOLO BOCCACCI (Persona)

Prodotto

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular breeding (Rivista)

Insieme di parole chiave di

• Parole chiave di "Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium-mediated transformation." (Insieme di parole chiave)