http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56821
Genetic variability and population structure of Grapevine virus A coat protein gene from naturally infected Italian vines (Articolo in rivista)
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- Genetic variability and population structure of Grapevine virus A coat protein gene from naturally infected Italian vines (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s10658-007-9202-0 (literal)
- Alternative label
Murolo S., Romanazzi G., Rowhani A., Minafra A., La Notte P., Branzanti M.B., Savino V. (2007)
Genetic variability and population structure of Grapevine virus A coat protein gene from naturally infected Italian vines
in European journal of plant pathology
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Murolo S., Romanazzi G., Rowhani A., Minafra A., La Notte P., Branzanti M.B., Savino V. (literal)
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- Pagina fine
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- http://www.springerlink.com/content/l313t1un31577307/fulltext.pdf (literal)
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- Rivista
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- IMPACT FACTOR 2007: 1.482 (I.F. 2008 non é ancora stato pubblicato dall'ISI WOK ) (literal)
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- Descrizione della variabilità molecolare del gene CP del virus A della vite da vigneti dellItalia centro-meridionale effettuata mediante RT-PCR e analisi di restrizione e sequenziamento dei prodotti PCR (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1) Dipartimento di Scienze Ambientali e delle Produzioni Vegetali, Università Politecnica delle Marche, Ancona;
2) Rowhani: Department of Plant Pathology, University of California, Davis, CA, USA;
3) CNR, Istituto di Virologia Vegetale U.O.S. di Bari;
4) Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari (literal)
- Titolo
- Genetic variability and population structure of Grapevine virus A coat protein gene from naturally infected Italian vines (literal)
- Abstract
- Grapevine virus A is considered one of the viruses associated with rugose wood one of the important diseases of grapevine. Thirty-seven GVA isolates collected from grapevine cultivars from Marche, Apulia and Campania, were subjected to molecular characterization. The genetic and population diversities were studied in the coat protein gene by RT-PCR-RFLP analysis with three restriction enzymes, and nucleotide sequencing. A new primer pair allowing to amplify the whole CP gene (621 bp) was developed. RFLP with AciI yielded the highest number of variants in GVA isolates, showing 7 different simple profiles Complex profiles were also found, and the most common variant combination was A+B in 39% of isolates. The analysis of GVA sequences confirmed the presence of plants infected with more than one GVA variant and suggested that RFLP is suitable to evaluate population diversity of GVA and to carry on a screening of different haplotypes. In the same vineyards under examination, the distribution of RFLP profiles and the phylogenetic analysis were not correlated with the location of infected plants, showing the presence of GVA population with genetic diversity in the average with those of RNA viruses. (literal)
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