Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1139/W08-010 (literal)

Alternative label

• Galetto, L., Fletcher, J., Bosco, D., Turina, M., Wayadande, A., Marzachi', C. (2008)
  Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate.
  in Canadian journal of microbiology (Print); National Research Council Of Canada-Nrc Research Press, Ottawa (Canada)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Galetto, L., Fletcher, J., Bosco, D., Turina, M., Wayadande, A., Marzachi', C. (literal)

Pagina inizio

• 341 (literal)

Pagina fine

• 351 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 54 (literal)

Rivista

• Canadian journal of microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• DOI: 10.1139/W08-010 IMPACT FACTOR 2007: 1.286 (I.F. 2008 non é ancora stato pubblicato dall'ISI WOK ) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 11 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• GL,TM, MC: Istituto di Virologia Vegetale, CNR, Torino, Italy GL,BD: Università degli Studi di Torino, Facoltà di Agraria, Di.Va.P.R.A., Entomologia e Zoologia Applicate all’Ambiente “Carlo Vidano”, Grugliasco, Torino, Italy. FJ,WA: Oklahoma State University, Department of Entomology & Plant Pathology, Stillwater OK, U.S. (literal)

Titolo

• Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate. (literal)

Abstract

• To characterize potentially important surface-exposed proteins of the phytoplasma causing chrysanthemum yellows (CY), new primers were designed based on the conserved regions of 3 membrane protein genes of the completely sequenced onion yellows and aster yellows witches’ broom phytoplasmas and were used to amplify CY DNA. The CY genes secY, amp, and artI, encoding the protein translocase subunit SecY, the antigenic membrane protein Amp and the arginine transporter ArtI, respectively, were cloned and completely sequenced. Alignment of CY-specific secY sequences with the corresponding genes of other phytoplasmas confirmed the 16S rDNA-based classification, while amp sequences were highly variable within the ‘Candidatus Phytoplasma asteris’. Five CY partial sequences were cloned into the pRSetC expression vector, and 3 of the encoded protein fragments (Amp 64/651, Amp 64/224, ArtI 131/512) were expressed as fusion antigens for the production of CY-specific polyclonal antibodies (A416 against Amp 64/224; A407 against ArtI 131/512). A416 recognized, in Western blots, the full-length Amp from CY-infected plants (periwinkle, daisy) and insect vectors (Euscelidius variegatus, Macrosteles quadripunctulatus). A416 also reacted to European aster yellows, to primula yellows phytoplasmas, to northern Italian strains of ‘Ca. Phytoplasma asteris’ from lettuce and gladiolus, but it did not react to American aster yellows phytoplasma. (literal)

Editore

• National Research Council Of Canada-Nrc Research Press (Editore)

Prodotto di

• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)

Autore CNR

• Luciana GALETTO (Persona)
• CRISTINA MARZACHI' (Unità di personale interno)
• MASSIMO TURINA (Unità di personale interno)
• Domenico Bosco (Persona)

Insieme di parole chiave

• Parole chiave di "Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MASSIMO TURINA (Unità di personale interno)
• CRISTINA MARZACHI' (Unità di personale interno)
• Domenico Bosco (Persona)
• Luciana GALETTO (Persona)

Prodotto

• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Canadian journal of microbiology (Rivista)

Editore di

• National Research Council Of Canada-Nrc Research Press (Editore)

Insieme di parole chiave di

• Parole chiave di "Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate." (Insieme di parole chiave)