Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus3 (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus3 (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00705-008-0100-3 (literal)

Alternative label

• Orecchia M.; Nolke G.; Saldarelli P.; Dell'Orco M.; Uhde-Holzem K.; Sack M.; Martelli G.P.; Fischer R.; Schillberg S. (2008)
  Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus3
  in Archives of virology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Orecchia M.; Nolke G.; Saldarelli P.; Dell'Orco M.; Uhde-Holzem K.; Sack M.; Martelli G.P.; Fischer R.; Schillberg S. (literal)

Pagina inizio

• 1075 (literal)

Pagina fine

• 1084 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Il lavoro descrive la selezione di un anticorpo ricombinante (scFv) specifico per il virus GLRaV-3 e la sua caratterizzazione. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 153 (literal)

Rivista

• Archives of virology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• IMPACT FACTOR 2007: 1.839 (I.F. 2008 non é ancora stato pubblicato dall'ISI WOK ) (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• * Institute for Molecular Biotechnology (Biology VII), RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany 1Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany (literal)

Titolo

• Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus3 (literal)

Abstract

• ABSTRACT: Pathogen-specific recombinant antibodies have been used to characterize pathogen infections and to engineer resistance in crops. We selected a single-chain antibody fragment (scFvLR3cp-1) specific for the coat protein of grapevine leafroll-associated virus 3 (GLRaV-3), one of the agents of grapevine leafroll (GLR) disease, from a phage display library. The antibody binds specifically to the entire length of GLRaV-3 particles and has a highbinding affinity value (KD) of 42 nM. The amino acid motif AQEPPRQ located at the N terminus of the GLRaV-3 coat protein was identified as the antibody-binding epitome by PEPSCAN analysis. To evaluate scFv stability in the reducing environment of the plant cell cytosol, transient expression assays were performed using Nicotiana benthamiana as a model plant. Capture ELISA demonstrated that the scFv fragment was produced and retained its antigenbinding capacity in the plant cytosol. Further functional assays showed that scFvLR3cp-1 binds with high specificità to at least four members of the family Closteroviridae. Therefore, the GLRaV-3-specific scFv fragment could be an ideal candidate for mediating broad-spectrum virus resistance if produced in transgenic grapevine plants. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• GIOVANNI MARTELLI (Unità di personale esterno)
• PASQUALE SALDARELLI (Persona)

Insieme di parole chiave

• Parole chiave di "Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus3" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• PASQUALE SALDARELLI (Persona)
• GIOVANNI MARTELLI (Unità di personale esterno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Archives of virology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus3" (Insieme di parole chiave)