Production of polyclonal and monoclonal antibodies specific against membrane proteins of “Candidatus Phytoplasma asteris”, chrysanthemum yellows isolate (CY)) (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Production of polyclonal and monoclonal antibodies specific against membrane proteins of “Candidatus Phytoplasma asteris”, chrysanthemum yellows isolate (CY)) (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Galetto, L., (1) Bosco, D., (2) Fletcher, J., Marzachì, C. (2007)
  Production of polyclonal and monoclonal antibodies specific against membrane proteins of “Candidatus Phytoplasma asteris”, chrysanthemum yellows isolate (CY))
  in Bulletin of insectology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Galetto, L., (1) Bosco, D., (2) Fletcher, J., Marzachì, C. (literal)

Pagina inizio

• 211 (literal)

Pagina fine

• 212 (literal)

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• This work was funded by MIUR under the FIRB grant RBAU014S8A (literal)

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• 60(2) (literal)

Rivista

• Bulletin of insectology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• The molecular reagents produced in this work are currently used to study the role of membrane proteins in the interaction between CY and its plant and vector hosts. (literal)

Note

• ISI Web of Science (WOS) (literal)

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• (1) Università degli Studi di Torino, Facoltà di Agraria, Di.Va.P.R.A., Entomologia e Zoologia Applicate all’Ambiente “Carlo Vidano”, Via L. Da Vinci 44, Grugliasco (TO) 10095, Italy. (2) Oklahoma State University, Department of Entomology & Plant Pathology, Noble Research Center, Stillwater OK 74078, U.S. (literal)

Titolo

• Production of polyclonal and monoclonal antibodies specific against membrane proteins of “Candidatus Phytoplasma asteris”, chrysanthemum yellows isolate (CY)) (literal)

Abstract

• Three genes encoding membrane proteins of chrysanthemum yellows phytoplasma were cloned and sequenced: secY, amp and artI encoding the protein translocase subunit SecY, the antigenic membrane protein Amp and the arginine transporter ArtI, re-spectively. Alignments of CY-specific secY sequences with the corresponding genes of other phytoplasmas confirmed the 16SrDNA-based classification, while Amp sequences resulted highly variable. Five CY partial sequences were cloned into pRSetC expression vector and two (Amp 64-224 and ArtI 131-512) were expressed as fusion antigens in E. coli for the production of CY-specific polyclonal antisera (A416 against Amp 64-224; A407 against ArtI 131-512) and monoclonal antibodies. A416 recognized, in Western blots, the full length Amp from CY-infected plants (periwinkle, daisy) and insect vectors (Euscelidius variegatus, Macrosteles quadripunctulatus). A416 also reacted to European aster yellows (EAY) and primula yellows (PY) phy-toplasmas and to Northern Italian strains of “Ca. P. asteris” from lettuce (LY163) and gladiolus (GLA), but not to American aster yellows (AAY) and clover phyllody (CPh) phytoplasmas. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• Luciana GALETTO (Persona)
• CRISTINA MARZACHI' (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Production of polyclonal and monoclonal antibodies specific against membrane proteins of “Candidatus Phytoplasma asteris”, chrysanthemum yellows isolate (CY))" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CRISTINA MARZACHI' (Unità di personale interno)
• Luciana GALETTO (Persona)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Bulletin of insectology (Rivista)

Insieme di parole chiave di

• Keywords of "Production of polyclonal and monoclonal antibodies specific against membrane proteins of “Candidatus Phytoplasma asteris”, chrysanthemum yellows isolate (CY))" (Insieme di parole chiave)