http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56703

A viroid RNA with a specific structural motif inhibits chloroplast development (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

A viroid RNA with a specific structural motif inhibits chloroplast development (Articolo in rivista) (literal)

Anno

2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1105/tpc.106.049775 (literal)

Alternative label

Maria-Elena Rodio (a); Sonia Delgado (b); Angelo De Stradis (a) Mar?a-Dolores Gomez (b); Ricardo Flores (b); Francesco Di Serio (a) (2007)
A viroid RNA with a specific structural motif inhibits chloroplast development
in The Plant cell; American Society Of Plant Biologists, Rockville (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Maria-Elena Rodio (a); Sonia Delgado (b); Angelo De Stradis (a) Mar?a-Dolores Gomez (b); Ricardo Flores (b); Francesco Di Serio (a) (literal)

Pagina inizio

3610 (literal)

Pagina fine

3616 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

Supplemental Data http://www.plantcell.org/cgi/content/full/tpc.106.049775/DC1 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.plantcell.org/content/19/11/3610.full.pdf+html?sid=6b3a4d16-cdff-423f-af9a-d9d725730f48 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

19 (literal)

Rivista

?The ?Plant cell (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

17 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

(a) Dipartimento di Protezione delle Piante e Microbiologia Applicata, Universita` degli Studi and Istituto di Virologia Vegetale del Consiglio Nazionale delle Ricerche, Sezione di Bari, 70126 Bari, Italy (b) Instituto de Biolog?´a Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cient?´ficas, Universidad Polite´ cnica de Valencia, 46022 Valencia, Spain (literal)

Titolo

A viroid RNA with a specific structural motif inhibits chloroplast development (literal)

Abstract

Peach latent mosaic viroid (PLMVd) is a chloroplast-replicating RNA that propagates in its natural host, peach (Prunus persica), as a complex mixture of variants, some of which are endowed with specific structural and pathogenic properties. This is the case of variant PC-C40, with an insertion of 12 to 13 nucleotides that folds into a hairpin capped by a U-rich loop, which is responsible for an albino-variegated phenotype known as peach calico (PC). We have applied a combination of ultrastructural, biochemical, and molecular approaches to dissect the pathogenic effects of PC-C40. Albino sectors of leaves infected with variant PC-C40 presented palisade cells that did not completely differentiate into a columnar layer and altered plastids with irregular shape and size and with rudimentary thylakoids, resembling proplastids. Furthermore, impaired processing and accumulation of plastid rRNAs and, consequently, of the plastid translation machinery was observed in the albino sectors of leaves infected with variant PC-C40 but not in the adjacent green areas or in leaves infected by mosaic-inducing or latent variants (including PC-C40D, in which the 12- to 13-nucleotide insertion was deleted). Protein gel blot and RT-PCR analyses showed that the altered plastids support the import of nucleus-encoded proteins, including a chloroplast RNA polymerase, the transcripts of which were detected. RNA gel blot and in situ hybridizations revealed that PLMVd replicates in the albino leaf sectors and that it can invade the shoot apical meristem and induce alterations in proplastids, bypassing the RNA surveillance system that restricts the entry of a nucleus-replicating viroid and most RNA viruses. Therefore, a non-protein-coding RNA with a specific structural motif can interfere with an early step of the chloroplast developmental program, leading ultimately to an albino-variegated phenotype resembling that of certain variegated mutants in which plastid rRNA maturation is also impaired. Our results highlight the potential of viroids for further dissection of RNA trafficking and pathogenesis in plants. (literal)

Editore