http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56684

Partial characterization of two divergent variants of Grapevine leafroll-associated virus 4 (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Partial characterization of two divergent variants of Grapevine leafroll-associated virus 4 (Articolo in rivista) (literal)

Anno

2006-01-01T00:00:00+01:00 (literal)

Alternative label

SALDARELLI P.; CORNUET P.; VIGNE E.; TALAS, F; BRONNEKANT I.; DRIDI A.M.; ANDRE-LINK P.; BOSCIA D.; GUGERLI P.; FUCHS M.; MARTELLI, J.P. (2006)
Partial characterization of two divergent variants of Grapevine leafroll-associated virus 4
in Journal of plant pathology (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

SALDARELLI P.; CORNUET P.; VIGNE E.; TALAS, F; BRONNEKANT I.; DRIDI A.M.; ANDRE-LINK P.; BOSCIA D.; GUGERLI P.; FUCHS M.; MARTELLI, J.P. (literal)

Pagina inizio

203 (literal)

Pagina fine

214 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://sipav.org/main/jpp/index.php/jpp/article/view/864/650 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

88 (literal)

Rivista

Journal of plant pathology (Rivista)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Istituto di Virologia Vegetale del CNR, sezione di Bari, Via Amendola 165/A, 70126 Bari, Italy Institut National de la Recherche Agronomique, Unité Mixte de Recherche 'Santé de la Vigne et Qualité des Vins', INRA-Université Louis Pasteur, Laboratoire de Virologie, 28 Rue de Herrlisheim, 68021 Colmar, France Agroscope, Federal Agricultural Research Station of Changins, P.O. Box 254, 1260 Nyon 1, Switzerland Istituto Agronomico Mediterraneo, Via Ceglie 9, 70010, Valenzano (Bari), Italy (literal)

Titolo

Partial characterization of two divergent variants of Grapevine leafroll-associated virus 4 (literal)

Abstract

Two non mechanically transmissible viruses with filamentous closterovirus-like particles were extracted and partially characterized from leafroll-affected grapevine accessions of cv Koussan from Turkey (Y253) and cv Koudsi from Israel (Y252), both from the reference collection of grapevine viruses and virus-like diseases at the Institut National de la Recherche Agronomique (INRA) in Colmar, France. These viruses, denoted Y253-TK and Y252-IL, were independently investigated in France (INRA-Colmar) and Italy (DPPMA). Y253-TK did not react in DAS-ELISA or RT-PCR assays that would have detected any of the Grapevine leafroll-associated viruses (GLRaVs) described so far; Y252-IL did give weak and inconsistent positive reactions in IEM only with an antiserum to strain LR106 of Grapevine leafroll-associated virus 4 (GLRaV-4). A polyclonal antiserum to isolate Y252-IL decorated the homologous particles and, those of Y253-TK and, at lower dilution, particles of isolate GLRaV-4 LR106. A segment of the heat shock protein p70 homologue (HSP70h) gene, was amplified by RT-PCR of RNA from denatured purified particles of Y253-TK by using a set of degenerate primers. The 183 amino acid polypeptide deduced from the 549 bp HSP70h gene fragment showed a high degree of identity with the cognate genes of GLRaV-4 (95%), GLRaV-5 (91%), GLRaV-6 (84%), and GLRaV-9 (90%), but low identity with those of other GLRaVs (33-35%). ne HSP70h of isolates Y252-IL and LR106 showed 95% amino acid identity. The coat protein sequence of isolates Y253-TK showed 99 and 94% amino acid identity with those of isolates Y252-IL and LR106, respectively, with changes concentrated in the N-terminus, a feature that might have a bearing on the reaction with their heterologous antisera. Based on serological and molecular data, it was concluded that Y253-TK and Y252-IL are distinct, though similar, isolates of GLRaV-4. In two independent surveys in which 110 and 320 grapevine accessions from germplasm collections at Colmar and Bari, respectively, were examined by DAS-ELISA, the antisera As-Y253-TK and As-Y252-IL cross-reacted with the homologous and hererologous viruses. Whereas As-Y253-TK did not react with any of the other accessions from the French collection tested, As-Y252-IL recognized a virus present in a total of eight grapevine accessions from the Mediterranean area, grown in the Italian germplasm collection. (literal)

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