Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Alternative label

• Di Serio F. 1, Schob H. 2, Iglesias A. 3, Tarina C. 4, Bouldoires E. 5, Meins F. 6. (2001)
  Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Di Serio F. 1, Schob H. 2, Iglesias A. 3, Tarina C. 4, Bouldoires E. 5, Meins F. 6. (literal)

Pagina inizio

• 6506 (literal)

Pagina fine

• 6510 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• I meccanismi molecolari coinvolti nel PTGS e nell'ASGS risultano di notevole interesse, soprattutto in considerazione della recente scoperta nella maggior parte degli organismi della regolazione di geni endogeni mediata da \"microRNAs\" e da \"small interfering RNAs\". Il presente lavoro è stato citato in articoli pubblicati nelle seguenti riviste ISI: The EMBO Journal, Gene and Development, Journal of Virology, The Plant Cell, PNAS, Plant Physiology, Plant Mol. Biol., Mol. Plant Microbe Interaction, Plant Journal, Molecular Plant Pathology, Clinical and Experimental Pharmacology and Physiology, Plant Cell and Environment, World Journal of Gastroenterology (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 98 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Si dimostra che il silenziamento di due geni di tabacco mediato da transgeni in orientazione antisenso (ASGS) è post-trascrizionale e, per la prima volta e con un adeguato sistema sperimentle, che l'ASGS è generato da un meccanismo molecolare, almeno in parte, simile a quello che determina il silenziamento genico mediato da transgeni in orientazione senso (post-transcription gene silencing, PTGS). Infatti, virus vegetali noti per codificare proteine in grado di sopprimere il PTGS inibiscono anche l'ASGS. Inoltre, in piante silenziate mediante ASGS si individuano RNA di piccole dimensioni (22-25 nt), omologhi e complementari al gene silenziato, che generalmente sono considerati marcatori molecolari del PTGS. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Istituto di Virologia Vegetale, sezione di Bari, Via Amendola 165/A, 70 126 Bari, Italia. 2,3,4,5,6. Friedrich Miescher Institute, A branch of the Novertis Reserch Foundation, Maulbeerstrasse 66, Ch-4058 Basel, Switzerland (literal)

Titolo

• Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs (literal)

Abstract

• Antisense-mediated gene silencing (ASGS) and posttranscriptional gene silencing (PTGS) with sense transgenes markedly reduce the steady-state mRNA levels of endogenous genes similar in transcribed sequence. RNase protection assays established that silencing in tobacco plants transformed with plant-defense-related class I sense and antisense chitinase (CHN) transgenes is at the posttranscriptional level. Infection of tobacco plants with cucumber mosaic virus strain FN and a necrotizing strain of potato virus Y, but not with potato virus X, effectively suppressed PTGS and ASGS of both the transgenes and homologous endogenes. This suggests that ASGS and PTGS share components associated with initiation and maintenance of the silent state. Small, ca. 25-nt RNAs (smRNA) of both polarities were associated with PTGS and ASGS in CHN transformants as reported for PTGS in other transgenic plants and for RNA interference in Drosophila. Similar results were obtained with an antisense class I beta-1,3-glucanase transformant showing that viral suppression and smRNAs are a more general feature of ASGS. Several current models hold that diverse signals lead to production of double-stranded RNAs, which are processed to smRNAs that then trigger PTGS. Our results provide direct evidence for mechanistic links between ASGS and PTGS and suggest that ASGS could join a common PTGS pathway at the double-stranded RNA step. (literal)

Autore CNR

• FRANCESCO DI SERIO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• FRANCESCO DI SERIO (Unità di personale interno)

Insieme di parole chiave di

• Keywords of "Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs" (Insieme di parole chiave)