Kinetics of rhodopsin and its role in regulating recovery and reproducibility of ros photoresponse (Articolo in rivista)

Type
Label
  • Kinetics of rhodopsin and its role in regulating recovery and reproducibility of ros photoresponse (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1371/journal.pcbi.1001031 (literal)
Alternative label
  • G. Caruso (1); P. Bisegna (2); L. Lenoci (3); D. Andreucci (4); V. Vsevolod Guverich (3); H.E. Hamm (3); E. DiBenedetto (5) (2010)
    Kinetics of rhodopsin and its role in regulating recovery and reproducibility of ros photoresponse
    in PLoS computational biology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • G. Caruso (1); P. Bisegna (2); L. Lenoci (3); D. Andreucci (4); V. Vsevolod Guverich (3); H.E. Hamm (3); E. DiBenedetto (5) (literal)
Pagina inizio
  • e1001031 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 6 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 15 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1) Istituto per le Tecnologie della Costruzione - Consiglio Nazionale delle Ricerche 2) Dipartimento di Ingegneria Civile - Università di Roma \"Tor Vergata\" 3) Department of Farmacology - Vanderbilt University, TN (USA) 4) Dipartimento di Metodi e Modelli Matematici per le Scienze Applicate - Università di Roma \"La Sapienza\" 5) Department of Mathematics - Vanderbilt University, TN (USA) (literal)
Titolo
  • Kinetics of rhodopsin and its role in regulating recovery and reproducibility of ros photoresponse (literal)
Abstract
  • The single photon response (SPR) in vertebrate phototransduction is regulated by the dynamics of R* during its lifetime, including the random number of phosphorylations, the catalytic activity and the random sojourn time at each phosphorylation level. Because of this randomness the electrical responses are expected to be inherently variable. However the SPR is highly reproducible. The mechanisms that confer to the SPR such a low variability are not completely understood. The kinetics of rhodopsin deactivation is investigated by a Continuous Time Markov Chain (CTMC) based on the biochemistry of rhodopsin activation and deactivation, interfaced with a spatio-temporal model of phototransduction. The model parameters are extracted from the photoresponse data of both wild type and mutant mice, having variable numbers of phosphorylation sites and, with the same set of parameters, the model reproduces both WT and mutant responses. The sources of variability are dissected into its components, by asking whether a random number of turnoff steps, a random sojourn time between steps, or both, give rise to the known variability. The model shows that only the randomness of the sojourn times in each of the phosphorylated states contributes to the Coefficient of Variation (CV) of the response, whereas the randomness of the number of R* turnoff steps has a negligible effect. These results counter the view that the larger the number of decay steps of R*, the more stable the photoresponse is. Our results indicate that R* shutoff is responsible for the variability of the photoresponse, while the diffusion of the second messengers acts as a variability suppressor. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it