Aggregation/fibrillogenesis of recombinant human prion protein and Gerstmann-Straussler_Scheinker disease peptides in the presence of metal ions. (Articolo in rivista)

Type
Label
  • Aggregation/fibrillogenesis of recombinant human prion protein and Gerstmann-Straussler_Scheinker disease peptides in the presence of metal ions. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/bi0601454 (literal)
Alternative label
  • RICCHELLI F.; BUGGIO R.; DRAGO D.; FORLONI G.; NEGRO A.; TOGNON G.; ZATTA P. (2006)
    Aggregation/fibrillogenesis of recombinant human prion protein and Gerstmann-Straussler_Scheinker disease peptides in the presence of metal ions.
    in Biochemistry (Easton)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • RICCHELLI F.; BUGGIO R.; DRAGO D.; FORLONI G.; NEGRO A.; TOGNON G.; ZATTA P. (literal)
Pagina inizio
  • 6724 (literal)
Pagina fine
  • 6732 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 45 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 21 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR University (literal)
Titolo
  • Aggregation/fibrillogenesis of recombinant human prion protein and Gerstmann-Straussler_Scheinker disease peptides in the presence of metal ions. (literal)
Abstract
  • In this study we investigated the role of Cu2+, Mn2+, Zn2+, and Al3+ in inducing defective conformational rearrangements of the recombinant human prion protein (hPrP), which trigger aggregation and fibrillogenesis. The research was extended to the fragment of hPrP spanning residues 82-146, which was identified as a major component of the amyloid deposits in the brain of patients affected by Gerstmann- Stra¨ussler-Scheinker (GSS) disease. Variants of the 82-146 wild-type subunit [PrP-(82-146)wt] were also examined, including entirely, [PrP-(82-146)scr], and partially scrambled, [PrP-(82-146)106-126scr] and [PrP-(82-146)127-146scr], peptides. Al3+ strongly stimulated the conversion of native hPrP into the altered conformation, and its potency in inducing aggregation was very high. Despite a lower rate and extent of prion protein conversion into altered isoforms, however, Zn2+ was more efficient than Al3+ in promoting organization of hPrP aggregates into well-structured, amyloid-like fibrillar filaments, whereas Mn2+ delayed and Cu2+ prevented the process. GSS peptides underwent the fibrillogenesis process much faster than the full-length protein. The intrinsic ability of PrP-(82-146)wt to form fibrillar aggregates was exalted in the presence of Zn2+ and, to a lesser extent, of Al3+, whereas Cu2+ and Mn2+ inhibited the conversion of the peptide into amyloid fibrils. Amino acid substitution in the neurotoxic core (sequence 106-126) of the 82-146 fragment reduced its amyloidogenic potential. In this case, the stimulatory effect of Zn2+ was lower as compared to the wild-type peptide; on the contrary Al3+ and Mn2+ induced a higher propensity to fibrillation, which was ascribed to different binding modalities to GSS peptides. In all cases, alteration of the 127-146 sequence strongly inhibited the fibrillogenesis process, thus suggesting that integrity of the C-terminal region was essential both to confer amyloidogenic properties on GSS peptides and to activate the stimulatory potential of the metal ions. (literal)
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