Effect of native and NH3 plasma-functionalized polymeric membranes on the gene expression profiles of primary hepatocytes (Articolo in rivista)

Type
Label
  • Effect of native and NH3 plasma-functionalized polymeric membranes on the gene expression profiles of primary hepatocytes (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/term.453 (literal)
Alternative label
  • Sanja Pavlica, Stephanie Schmitmeier, Pia Gloeckner, Antonella Piscioneri, Frank Peinemann, Knut Krohn, Michaela Siegmund-Schulz, Stefania Laera, Pietro Favia, Loredana De Bartolo, Augustinus Bader (2012)
    Effect of native and NH3 plasma-functionalized polymeric membranes on the gene expression profiles of primary hepatocytes
    in Journal of tissue engineering and regenerative medicine
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sanja Pavlica, Stephanie Schmitmeier, Pia Gloeckner, Antonella Piscioneri, Frank Peinemann, Knut Krohn, Michaela Siegmund-Schulz, Stefania Laera, Pietro Favia, Loredana De Bartolo, Augustinus Bader (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://onlinelibrary.wiley.com/doi/10.1002/term.453/abstract (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • DOI: 10.1002/term.453 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Centre for Biotechnology and Biomedicine, BBZ, University of Leipzig, Germany Institute of Membrane Technology, National Research Council of Italy, ITM-CNR, University of Calabria, Rende (CS), Italy Department of Chemistry, University of Bari, Italy IZKF, Core Unit DNA-Technologies, University of Leipzig, Germany Department of Pharmaceutical Technologies, University of Leipzig, Germany (literal)
Titolo
  • Effect of native and NH3 plasma-functionalized polymeric membranes on the gene expression profiles of primary hepatocytes (literal)
Abstract
  • Little is known about how cells respond to different biomaterials at the molecular level. Biomaterials could stimulate specific cellular responses at the molecular level, such as activation of signaling pathways that control gene activity involved in the maintenance, growth and functional regeneration of liver tissue in vitro. This aspect is an important step in liver tissue engineering. Currently, there are no data available concerning the modulation of cellular genomic response by using synthetic membranes in a bioartificial system. For the first time we investigated gene expression profiles of primary hepatocytes cultured on different substrates: collagen sandwich, native and NH3 plasma-grafted PEEK–WC–PU membranes. Gene expression in cell suspension prepared after cell isolation was used as a control. Generally, microarray data revealed that the expression of the majority of genes remained unchanged compared to the control. Among 31 000 genes, 52 were significantly changed: 20 were upregulated and 32 downregulated. There were similar changes in gene expression of hepatocytes cultured in the membranes and collagen sandwich. However, some genes involved in the cell proliferation and functional metabolic pathways are more expressed in cells cultured on the membranes and especially on the functionalized ones. Both membranes sustained liver functions at the molecular level, demonstrating their suitability for the reconstruction of liver and as a toxicogenomic tool to predict the liver response to novel drugs. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it