http://www.cnr.it/ontology/cnr/individuo/prodotto/ID55185

Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network (Articolo in rivista)

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Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network (Articolo in rivista) (literal)

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Alternative label

Sabrina Morelli, Antonella Piscioneri, Simona Salerno, Maria Rende, Carla Campana, Franco Tasselli, Anna di Vito, Giuseppina Giusi, Marcello Canonaco, Enrico Drioli and Loredana De Bartolo (2012)
Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network
in Journal of tissue engineering and regenerative medicine
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Sabrina Morelli, Antonella Piscioneri, Simona Salerno, Maria Rende, Carla Campana, Franco Tasselli, Anna di Vito, Giuseppina Giusi, Marcello Canonaco, Enrico Drioli and Loredana De Bartolo (literal)

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http://onlinelibrary.wiley.com/doi/10.1002/term.434/abstract;jsessionid=01D3E091D11A2B9B43FB5767FDA08BC8.f01t02 (literal)

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1Institute of Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, Cubo 17/C, 87030, Rende (CS), Italy 2Department of Chemical Engineering and Materials of University of Calabria, via P. Bucci Cubo 45/A, 87030 Rende (CS), Italy 3Comparative Neuroanatomy Laboratory, Department of Ecology, University of Calabria, via P. Bucci 4/B, 87030 Rende (CS), Italy (literal)

Titolo

Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network (literal)

Abstract

The selection of appropriate biomaterials that promote cellular adhesion and growth is particularly important for the in vitro reconstruction of neuronal network. This study focused on the development of new polymeric membranes in flat and tubular (hollow-fibre) configurations as novel biomaterials for neuronal outgrowth. Two membrane systems constituted by modified polyetheretherketone (PEEK-WC) and polyacrylonitrile (PAN) membranes were developed and used for the culture of hamster hippocampal neurons. We demonstrated that all investigated membranes supported the adhesion and growth of hippocampal neurons enhancing neuronal differentiation and neurite alignment. The differences in cell behaviours between cells cultured on flat and hollow-fibre (HF) membranes were highlighted by the quantitative analysis of neuronal marker fluorescence intensity, morphometric analysis, RTPCR analysis and also by metabolic activity measurements. In particular, the PAN HF membranes showed ideal growth culture conditions, guaranteeing adequate levels of metabolic features. Primary hippocampal cells cultured on PAN HF membranes were able to recreate in vitro a 3D neural tissue-like structure that, mimicking the hippocampal tissue, could be used as a tool for the study of natural and pathological neurobiological events. (literal)

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