http://www.cnr.it/ontology/cnr/individuo/prodotto/ID55090

Interactive functional poly(vinylidene fluoride) membranes with modulated Lysozyme affinity: a promising class of new interfaces for contactor crystallizers (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Interactive functional poly(vinylidene fluoride) membranes with modulated Lysozyme affinity: a promising class of new interfaces for contactor crystallizers (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1002/pi.2681 (literal)

Alternative label

Gugliuzza, A., Aceto, M., Drioli, E. (2009)
Interactive functional poly(vinylidene fluoride) membranes with modulated Lysozyme affinity: a promising class of new interfaces for contactor crystallizers
in Polymer international
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Gugliuzza, A., Aceto, M., Drioli, E. (literal)

Pagina inizio

1452 (literal)

Pagina fine

1464 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0126 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

58 (literal)

Rivista

Polymer international (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

DOI 10.1002/pi.2681 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Research Institute on Membrane Technology-National Research Council, ITM-CNR, Via Pietro Bucci 17/C, I-87030 Rende, Italy, Department of Pharmaceutical Science, University of Calabria, Via Pietro Bucci, I-87030 Rende, Italy Department of Chemical Engineering and Materials, University of Calabria, Via Pietro Bucci 42/A, I-87030 Rende, Italy (literal)

Titolo

Interactive functional poly(vinylidene fluoride) membranes with modulated Lysozyme affinity: a promising class of new interfaces for contactor crystallizers (literal)

Abstract

BACKGROUND: One of the challenges of current researches in biotechnological field is the achievement of regular and increasingly smaller protein crystals for genomics and biocatalyst applications. The membrane contactor-based methodology appears a time-effectiveness and economically competitive technology for accomplishing this target. RESULTS: Here, a new class of interactive polymeric interfaces enabling to nucleate massively smaller protein crystals in even shorter time through controlling attractive interactions is discussed. Specifically, the role of attractive interfacial forces between a model Lysozyme solution and modified poly(vinylidene fluoride) membranes has been examined. The insertion of amphiphilic motifs in fluorinated membranes allowed quicker agglomeration of protein crystals at the membrane surface reducing significantly the induction time to the protein nucleation. The chemical nature of the modifier permitted to modulate the membrane affinity to the Lysozyme, involving polar and non polar attractive interactions and preserving intrinsic structural features, transport properties and hydrophobic character of the interfaces, according to the basics of the membrane crystallization technology. The formation of critical nuclei was observed after 3 hours and micro-sized crystals were formed in less than 24 hours. CONCLUSION: The experimental evidences suggest these membranes as a promising class of interactive interfaces that may rapidly bring to advances in genomics research. (literal)

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