http://www.cnr.it/ontology/cnr/individuo/prodotto/ID54050
Fractionation and Characterization of Ultra-High Molar Mass Hyaluronan: 2. On-line Size Exclusion Chromatography Methods (Articolo in rivista)
- Type
- Label
- Fractionation and Characterization of Ultra-High Molar Mass Hyaluronan: 2. On-line Size Exclusion Chromatography Methods (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Mendichi R., Giacometti Schieroni A. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Titolo
- Fractionation and Characterization of Ultra-High Molar Mass Hyaluronan: 2. On-line Size Exclusion Chromatography Methods (literal)
- Abstract
- Present study concerns SEC fractionation of ultra-high molar mass
Hyaluronan. Problems in SEC of UHMM HA samples are the fractionation in
the columns and the calibration of the system. We have overcome the
calibration problem using absolute detectors, light scattering and
viscometer, on-line to the SEC system that do not need of calibration.
Shear degradation, concentration effects, viscous fingering, poor column
resolution and in general low reproducibility are the main difficulties
in SEC fractionation. A successful characterization of UHMM HA polymers
requires an optimization of the experimental protocol. Each step of the
experimental protocol should be performed methodically to obtain reliable
results. Commercially available SEC aqueous columns have not optimized
for UHMM polymers. Using an optimized SEC system shear degradation and
non-ideal SEC fractionation can occur when Mw of the HA sample is
approximately higher than three million. Also the dispersity index could
be underestimated. (literal)
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