http://www.cnr.it/ontology/cnr/individuo/prodotto/ID53293

OXIDASE ENZYME IMMOBILISATION THROUGH ELECTROPOLYMERISED FILMS TO ASSEMBLE BIOSENSORS FOR BATCH AND FLOW INJECTION ANALYSIS (Articolo in rivista)

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OXIDASE ENZYME IMMOBILISATION THROUGH ELECTROPOLYMERISED FILMS TO ASSEMBLE BIOSENSORS FOR BATCH AND FLOW INJECTION ANALYSIS (Articolo in rivista) (literal)

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Alternative label

Mihaela Badea , Antonella Curulli , Giuseppe Palleschi (2003)
OXIDASE ENZYME IMMOBILISATION THROUGH ELECTROPOLYMERISED FILMS TO ASSEMBLE BIOSENSORS FOR BATCH AND FLOW INJECTION ANALYSIS
in Biosensors & bioelectronics; ELSEVIER APPL SCI PUBL LTD,, barking Essex (Regno Unito)
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Il modello di biosensore proposto comporta una conoscenza approfondita non solo delle problematiche di analisi, ma anche uno studio approfondito dei materiali coinvolti (literal)

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Articolo su rivista internazionale ben conosciuta per lo sviluppo di nuovi modelli di biosensori. La novità sta nel fatto che si tratta di un sistema di semplice assemblaggio e di facile applicazione per biosensori screen printed da usare in continuo per analisi di tipo clinico o ambientale (literal)

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Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Rome, Italy CNR Istituto per lo Studio di Materiali Nanostrutturati Sezione Roma 2, Rome, Italy (literal)

Titolo

OXIDASE ENZYME IMMOBILISATION THROUGH ELECTROPOLYMERISED FILMS TO ASSEMBLE BIOSENSORS FOR BATCH AND FLOW INJECTION ANALYSIS (literal)

Abstract

Glucose oxidase, lactate oxidase, L-aminoacid oxidase and alcohol oxidase were immobilised on new films based on 2,6-dihydroxynaphtalene (2,6-DHN) copolymerised with 2-(4-aminophenyl)-ethylamine (AP-EA) onto the Pt electrodes. The electropolymerisation was performed by cyclic voltammetry. Different scan rates and scan potential ranges were investigated and selected according to the monomers used. These sensors were tested for hydrogen peroxide, ascorbic acid and acetaminophen by cyclic voltammetry and amperometry. The amperometric studies were carried out in batch as well as in a flow injection system (FIA). Analytical parameters such as reproducibility, interferences rejection, response time, buffer, storage and operational time of the sensors have been studied. The obtained films were also characterised by X-ray photoelectron spectroscopy (XPS). Different strategies for enzyme immobilisation were performed and discussed: enzyme entrapment in the film during the electropolymerisation and covalent attachment of the enzyme to the film via a carbodiimide (EDC) or glutaraldehyde. Different parameters were considered in order to optimise the immobilisation procedures. Results provide a guide to design high sensitive, stable and interference-free biosensors. In addition, studies were performed using these probes in an original flow injection system based on solenoidal valves. Sensor stability, life time and dynamic range were also optimised in these conditions (literal)

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