Micro-SERS spectroscopy for studying biological systems: an application to human spermatozoa (Scheda bibliografica)

Type

• Scheda bibliografica (Classe)
• Prodotto della ricerca (Classe)

Label

• Micro-SERS spectroscopy for studying biological systems: an application to human spermatozoa (Scheda bibliografica) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Alternative label

• Quagliano L. G., piacentini R., Silvestroni L., Palleschi S. (2002)
  Micro-SERS spectroscopy for studying biological systems: an application to human spermatozoa
  in Biophysical journal (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Quagliano L. G., piacentini R., Silvestroni L., Palleschi S. (literal)

Pagina inizio

• 39a (literal)

Rivista

• Biophysical journal (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• ISMN_CNR (literal)

Titolo

• Micro-SERS spectroscopy for studying biological systems: an application to human spermatozoa (literal)

Abstract

• Micro-SERS (Surface Enhanced Raman Scattering) from human spermatozoa on nanostructured silver deposited on semiconductor surfaces is reported. As a spectroscopic tool Raman technique gives detailed information about chemical composition, molecular conformation, interaction and functioning of biological molecules. Although many advantages make Raman spectroscopy a powerful technique for biological applications, it has not been widely applied yet. The small Raman cross-section, which results in a very low signal level and the strong fluorescence background, which is often observed in the spectra, sometimes made it impossible to obtain a good spectrum. Motivated by the SERS sensitivity to very small amounts of material and by its ability to quench the fluorescence, we are interested in the application of the SERS technique for studying complex biological systems, such as cells. Using nanometer-sized Ag particles deposited on GaAs surfaces as SERS-active substrate we have observed an enhancement of Raman signal from human spermatozoa. We have obtained structural information on extremely small amounts of biomaterials and at the same time evidence that the biomolecules are preserved on the surface. This work suggests micro-SERS spectroscopy as a promising technique to biological applications. (literal)

Prodotto di

• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)

Autore CNR

• LUCIA GIACINTA QUAGLIANO (Unità di personale interno)

Incoming links:

Prodotto

• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)

Autore CNR di

• LUCIA GIACINTA QUAGLIANO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biophysical journal (Rivista)