http://www.cnr.it/ontology/cnr/individuo/prodotto/ID52296
Photobleaching-free infrared near-field microscopy localizes molecules in neurons (Articolo in rivista)
- Type
- Label
- Photobleaching-free infrared near-field microscopy localizes molecules in neurons (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1063/1.3003567 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Generosi J.1; Margaritondo G.1; Kropf M.2; Hirling H.2; Catsicas S.2; Johnsson K.3; Tolk N.H.4; Piston D.W.5; A. Cricenti A.6 (literal)
- Pagina inizio
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- http://dx.doi.org/10.1063/1.3003567 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1 Institute of Physics of the Complex Matter, EPFL, 1015 Lausanne, Switzerland;
2 Cellular Neurobiology Laboratory, EPFL, 1015 Lausanne, Switzerland;
3 Protein Engineering Laboratory, EPFL, 1015 Lausanne, Switzerland;
4 Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee 31235, USA;
5 Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232, USA;
6 Istituto di Struttura della Materia, CNR, via Fosso del Cavaliere 100, 00133 Roma, Italy and Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee 31235, USA (literal)
- Titolo
- Photobleaching-free infrared near-field microscopy localizes molecules in neurons (literal)
- Abstract
- High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acidtype glutamate receptor clusters on hippocampal neurons. The GluR2 subunits were labeled with the die Alexa 488 and high-resolution infrared micrographs were taken at 6.25 µm. The absence of photobleaching makes this approach suitable for a long-term observation and allows to localize different infrared-absorbing molecules over the complex background of other cell components. (literal)
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- Autore CNR
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