PC4/Tis7/IFRD1 stimulates skeletal muscle regeneration and is involved in myoblast differentiation as a regulator of MyoD and NF-kB (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• PC4/Tis7/IFRD1 stimulates skeletal muscle regeneration and is involved in myoblast differentiation as a regulator of MyoD and NF-kB (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1074/jbc.M110.162842 (literal)

Alternative label

• Micheli L.1*, Leonardi L.1*, Conti F.1*, Maresca G.1, Colazingari S.1, Mattei E.1, Lira S.A.2, Farioli-Vecchioli S.1, Caruso M.1, Tirone F.1 (2010)
  PC4/Tis7/IFRD1 stimulates skeletal muscle regeneration and is involved in myoblast differentiation as a regulator of MyoD and NF-kB
  in Journal of biological chemistry (Online); American society for biochemistry and molecular biology, Baltimore (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Micheli L.1*, Leonardi L.1*, Conti F.1*, Maresca G.1, Colazingari S.1, Mattei E.1, Lira S.A.2, Farioli-Vecchioli S.1, Caruso M.1, Tirone F.1 (literal)

Pagina inizio

• 5691 (literal)

Pagina fine

• 5707 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• - Pubblicato in formato elettronico il 2 Decembre, 2010, doi: 10.1074/jbc.M110.162842 - Pubblicato in formato a stampa il 18 Febbraio, 2011 - The Journal of Biological Chemistry, Vol. 286, p. 5691-5707 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.jbc.org/content/286/7/5691 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 286 (literal)

Rivista

• Journal of biological chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 7 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 - Istituto di Neurobiologia e Medicina Molecolare, Consiglio Nazionale delle Ricerche, Fondazione S.Lucia, Via del Fosso di Fiorano 64, 00143, Rome, Italy; 2 - Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA. (literal)

Titolo

• PC4/Tis7/IFRD1 stimulates skeletal muscle regeneration and is involved in myoblast differentiation as a regulator of MyoD and NF-kB (literal)

Abstract

• In skeletal muscle cells the PC4 (Tis7/IFRD1) protein is known to function as a coactivator of MyoD by promoting the transcriptional activity of myocyte enhancer factor 2C (MEF2C). In this study we show that upregulation of PC4 in vivo in adult muscle significantly potentiates injury-induced regeneration by enhancing myogenesis. Conversely, we observe that PC4-silencing in myoblasts causes delayed exit from the cell cycle, accompanied by delayed differentiation, and show that such effect is MyoD-dependent. We provide evidence revealing a novel mechanism underlying the promyogenic actions of PC4, by which PC4 functions as a negative regulator of NF-kB, known to inhibit MyoD expression post-transcriptionally. In fact, upregulation of PC4 in primary myoblasts induces the deacetylation, and hence the inactivation and nuclear export of NF-kB p65, in concomitance with induction of MyoD expression. On the contrary, PC4-silencing in myoblasts induces the acetylation and nuclear import of p65, in parallel with a decrease of MyoD levels. We also observe that PC4 potentiates the inhibition of NF-kbeta transcriptional activity mediated by histone deacetylases (HDACs) and that PC4 is able to form trimolecular complexes with p65 and HDAC3. This suggests that PC4 stimulates deacetylation of p65 by favoring the recruitment of HDAC3 to p65. As a whole, these results indicate that PC4 plays a role in muscle differentiation by controlling the MyoD pathway through multiple mechanisms, and as such positively regulates regenerative myogenesis. (literal)

Editore

• American society for biochemistry and molecular biology (Editore)

Prodotto di

• Sviluppo, Differenziamento e Trasformazione Cellulare (SV.P15.006.001) (Modulo)
• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Autore CNR

• MAURIZIA CARUSO (Persona)
• FELICE TIRONE (Unità di personale interno)
• LAURA MICHELI (Unità di personale interno)
• STEFANO FARIOLI VECCHIOLI (Unità di personale interno)
• ELISABETTA MATTEI (Persona)
• LUCA LEONARDI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "PC4/Tis7/IFRD1 stimulates skeletal muscle regeneration and is involved in myoblast differentiation as a regulator of MyoD and NF-kB" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• FELICE TIRONE (Unità di personale interno)
• LUCA LEONARDI (Unità di personale interno)
• MAURIZIA CARUSO (Persona)
• STEFANO FARIOLI VECCHIOLI (Unità di personale interno)
• ELISABETTA MATTEI (Persona)
• LAURA MICHELI (Unità di personale interno)

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Sviluppo, Differenziamento e Trasformazione Cellulare (SV.P15.006.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of biological chemistry (Rivista)

Editore di

• American society for biochemistry and molecular biology (Editore)

Insieme di parole chiave di

• Keywords of "PC4/Tis7/IFRD1 stimulates skeletal muscle regeneration and is involved in myoblast differentiation as a regulator of MyoD and NF-kB" (Insieme di parole chiave)