http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4987

Rapid constitutive and ligand-activated endocytic trafficking of P2X receptor (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Rapid constitutive and ligand-activated endocytic trafficking of P2X receptor (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1111/j.1471-4159.2009.06029.x (literal)

Alternative label

Vacca F. 1,2; Giustizieri M. 3;Ciotti M.T. 4; Mercuri N.B. 3,4;Volonté C. 1,4 (2009)
Rapid constitutive and ligand-activated endocytic trafficking of P2X receptor
in Journal of neurochemistry
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Vacca F. 1,2; Giustizieri M. 3;Ciotti M.T. 4; Mercuri N.B. 3,4;Volonté C. 1,4 (literal)

Pagina inizio

1031 (literal)

Pagina fine

1041 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

IF 3.999 SCI-JCR 2009 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

109 (literal)

Rivista

Journal of neurochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

4 (literal)

Note

PubMe (literal)
Scopu (literal)
ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1 - Laboratory of Cellular Neurobiology, Santa Lucia Foundation, Rome, Italy; 2 - Department of Biochemistry, University of Geneva, Geneva, Switwerland; 3 - Laboratory of Experimental Neurology, Santa Lucia Foundation, Rome, Italy; 4 - CNR, Institute of Neurobiology & Molecular Medicine, Rome, Italy; 5 - Department of Neuroscience, University of Rome 'Tor Vergata', Rome, Italy (literal)

Titolo

Rapid constitutive and ligand-activated endocytic trafficking of P2X receptor (literal)

Abstract

P2X receptors mediate a variety of physiological actions, including smooth muscle contraction, neuro-endocrine secretion and synaptic transmission. Among P2X receptors, the P2X(3) subtype is expressed in sensory neurons of dorsal root- and trigeminal-ganglia, where it performs a well-recognized role in sensory and pain transmission. Recent evidence indicates that the strength of P2X(3)-mediated responses is modulated in vivo by altering the number of receptors at the plasma membrane. In the present study, we investigate the trafficking properties of P2X(3) receptor in transfected HEK293 cells and in primary cultures of dorsal root ganglion neurons, finding that P2X(3) receptor undergoes rapid constitutive and cholesterol-dependent endocytosis. We also show that endocytosis is accompanied by preferential targeting of the receptor to late endosomes/lysosomes, with subsequent degradation. Furthermore, we observe that at steady state the receptor localizes predominantly in lamp1-positive intracellular structures, with a minor fraction present at the plasma membrane. Finally, the level of functional receptor expressed on the cell surface is rapidly up-regulated in response to agonist stimulation, which also augments receptor endocytosis. The findings presented in this work underscore a very dynamic trafficking behavior of P2X(3) receptor and disclose a possible mechanism for the rapid modulation of ATP-mediated responses potentially relevant during physiological and pathological conditions. (literal)

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