Altered Intracellular Calcium Fluxes in Pancreatic Cancer Induced Diabetes Mellitus: Relevance of the S100A8 N-Terminal Peptide (NT-S100A8) (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Altered Intracellular Calcium Fluxes in Pancreatic Cancer Induced Diabetes Mellitus: Relevance of the S100A8 N-Terminal Peptide (NT-S100A8) (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/jcp.22355 (literal)

Alternative label

• Basso D.; Greco E.; Padoan A.; Fogar P.; Scorzeto M.; Fadi E.; Bozzato D.; Moz S.; Navaglia F.; Zambon C.; Seraglia R.; De Carlo E.; Valerio A.; Reggiani C.; Pedrazzoli S.; Plebani M. (2011)
  Altered Intracellular Calcium Fluxes in Pancreatic Cancer Induced Diabetes Mellitus: Relevance of the S100A8 N-Terminal Peptide (NT-S100A8)
  in Journal of cellular physiology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Basso D.; Greco E.; Padoan A.; Fogar P.; Scorzeto M.; Fadi E.; Bozzato D.; Moz S.; Navaglia F.; Zambon C.; Seraglia R.; De Carlo E.; Valerio A.; Reggiani C.; Pedrazzoli S.; Plebani M. (literal)

Pagina inizio

• 456 (literal)

Pagina fine

• 468 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://onlinelibrary.wiley.com/doi/10.1002/jcp.22355/abstract (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 226 (literal)

Rivista

• Journal of cellular physiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• pubblicazione scientifica (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1,4,9,16 : Department of Laboratory Medicine, University of Padua, Padua, Italy / 2,3,6,7,8,16 : Department of Diagnostic Science and Special Therapies, University of Padua, Padua, Italy / 5,14 : Department of Anatomy and Physiology, University of Padua, Padua, Italy / 10,12,15 : Department of Medical and Surgical Science, University of Padua, Padua, Italy / 11 : CNR, ISTM, Padua, Italy / 13 Department of Clinical and Experimental Medicine, University of Padua, Padua, Italy (literal)

Titolo

• Altered Intracellular Calcium Fluxes in Pancreatic Cancer Induced Diabetes Mellitus: Relevance of the S100A8 N-Terminal Peptide (NT-S100A8) (literal)

Abstract

• After isolating NT-S100A8 from pancreatic cancer (PC) tissue of diabetic patients, we verified whether this peptide alters PC cell growth and invasion and/or insulin release and [Ca(2+)](i) oscillations of insulin secreting cells and/or insulin signaling. BxPC3, Capan1, MiaPaCa2, Panc1 (PC cell lines) cell growth, and invasion were assessed in the absence or presence of 50, 200, and 500 nM NT-S100A8. In NT-S100A8 stimulated beta-TC6 (insulinoma cell line) culture medium, insulin and [Ca(2+)](i) were measured at 2, 3, 5, 10, 15, 30, and 60 min, and [Ca(2+)](i) oscillations were monitored (epifluorescence) for 3 min. Five hundred nanomolars NT-S100A8 stimulated BxPC3 cell growth only and dose dependently reduced MiaPaCa2 and Panc1 invasion. Five hundred nanomolars NT-S100A8 induced a rapid insulin release and enhanced beta-TC6 [Ca(2+)](i) oscillations after both one (F = 6.05, P < 0.01) and 2 min (F = 7.42, P < 0.01). In the presence of NT-S100A8, [Ca(2+)] in beta-TC6 culture medium significantly decreased with respect to control cells (F = 6.3, P < 0.01). NT-S100A8 did not counteract insulin induced phosphorylation of the insulin receptor. Akt and I kappa B-alpha, but it independently activated Akt and NF-kappa B signaling in PC cells. In conclusion, NT-S100A8 exerts a mild effect on PC cell growth, while it reduces PC cell invasion, possibly by Akt and NF-kappa B signaling, NT-S100A8 enhances [Ca(2+)](i) oscillations and insulin release, probably by inducing Ca(2+) influx from the extracellular space, but it does not interfere with insulin signaling. (literal)

Prodotto di

• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)
• Identificazione biomarcatori proteici per diabete, nefropatie e tumori mediante spettrometria di massa (PM.P06.008.001) (Modulo)
• Institute of Condensed Matter Chemistry and Energy Technologies (ICMATE) (Istituto)

Autore CNR

• ROBERTA SERAGLIA (Persona)

Insieme di parole chiave

• Keywords of "Altered Intracellular Calcium Fluxes in Pancreatic Cancer Induced Diabetes Mellitus: Relevance of the S100A8 N-Terminal Peptide (NT-S100A8)" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)
• Institute of Condensed Matter Chemistry and Energy Technologies (ICMATE) (Istituto)
• Identificazione biomarcatori proteici per diabete, nefropatie e tumori mediante spettrometria di massa (PM.P06.008.001) (Modulo)

Autore CNR di

• ROBERTA SERAGLIA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of cellular physiology (Rivista)

Insieme di parole chiave di

• Keywords of "Altered Intracellular Calcium Fluxes in Pancreatic Cancer Induced Diabetes Mellitus: Relevance of the S100A8 N-Terminal Peptide (NT-S100A8)" (Insieme di parole chiave)