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Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles (Articolo in rivista)
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- Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
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Bertazzo, A.; De Rosso, M.; Dalla Vedova, A.; Agnolin, F.; Flamini, R.; Traldi, P. (2010)
Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles
in Journal of mass spectrometry (Print)
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- Bertazzo, A.; De Rosso, M.; Dalla Vedova, A.; Agnolin, F.; Flamini, R.; Traldi, P. (literal)
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- DOI 10.1002/jms.1738 (literal)
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- ISI Web of Science (WOS) (literal)
- Titolo
- Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles (literal)
- Abstract
- Two Raboso cultivars, i.e. Raboso Veronese and Raboso Piave, are two black Vitis vinifera varieties. A genetic study suggested
that Raboso Veronese is the progeny of a spontaneous cross between Raboso Piave and Marzemina Bianca cultivars. Parental
relationships are usually investigated by genetic studies, which are effective to establish genetic linksamongdifferent cultivars.
Considering that proteome is the genome expression, in this article we evaluated the power of seed protein profiles obtained
by matrix-assisted laser desorption/ionization (MALDI)/MS for parentage investigation. The three cultivars lead to very similar
spectra with differences in the relative intensity of the most abundant species and the presence of very weak specific ions. In
order to evaluate the analytical significance of these aspects, the variability due to instrumental factors and due to different
harvesting areas and years of the same cultivars have been considered and measured by the calculation of discrepancy factor
values. On one hand, the results obtained can be considered a valid confirmation of the genomic findings,whereas on the other
hand, the results provide evidence for the ability of MALDI/MS to individuate minor differences in protein profiles of complex
proteinmixtures. (literal)
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