Femtomolar detection of autoantibodies by magnetic relaxation nanosensors (Articolo in rivista)

Type
Label
  • Femtomolar detection of autoantibodies by magnetic relaxation nanosensors (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.ab.2009.05.034 (literal)
Alternative label
  • Colombo, Miriam; Ronchi, Silvia; Monti, Diego; Corsi, Fabio; Trabucchi, Emilio; Prosperi, Davide (2009)
    Femtomolar detection of autoantibodies by magnetic relaxation nanosensors
    in Analytical biochemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Colombo, Miriam; Ronchi, Silvia; Monti, Diego; Corsi, Fabio; Trabucchi, Emilio; Prosperi, Davide (literal)
Pagina inizio
  • 96 (literal)
Pagina fine
  • 102 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • This work was Supported by \"Fondazione Romeo ed Enrica Invernizzi\" and the CNR-Regione Lombardia \"Mind in Italy\" project. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://dx.doi.org/10.1016/j.ab.2009.05.034 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 392 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • DOI: 10.1016/j.ab.2009.05.034 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Univ Milano Bicocca, Dipartimento Biotecnol & Biosci, I-20126 Milan, Italy (Colombo, M.; Prosperi, D.) 2. CNR, Ist Sci & Tecnol Mol, I-20138 Milan, Italy (Monti, D.; Prosperi, D.; Ronchi, S.) 3. Univ Milan, Dipartimento Sci Clin Luigi Sacco, I-20157 Milan, Italy (Corsi, F.; Trabucchi, E.) (literal)
Titolo
  • Femtomolar detection of autoantibodies by magnetic relaxation nanosensors (literal)
Abstract
  • The development of nanosystems applied to rapid and sensitive measurement of biomarkers in fluid samples is a current major goal in diagnostic biomedicine. In this article, we report the accurate and reliable detection of anti-HSA (human serum albumin) antibodies by protein-functionalized magnetic nanospherical probes due to the reversible alteration of their microaggregation state induced by protein antibody-specific interaction, sensed as changes in the T-2 relaxation time of surrounding water molecules. Once the optimal parameters were adjusted, the method proved to be very sensitive, providing concentration- and time-dependent responses. Furthermore, we demonstrate that the developed immunoassay is able to quantitatively determine the biomarker concentration from T-2 linear correlation, thereby supplying a rapid, yet accurate, assay with sensitivity in the femtomolar range. The high susceptibility and stability of these magnetic nanoparticles, as well as their accessible synthetic preparation, make these nanosensors a promising new tool for versatile and effective medical diagnostics. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it