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A fast screening MALDI method for the detection of cocaine and its metabolites in hair (Articolo in rivista)

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A fast screening MALDI method for the detection of cocaine and its metabolites in hair (Articolo in rivista) (literal)

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Susanna Vogliardi,a; Donata Favretto,a; Giampietro Frison,a; Santo Davide Ferrara,a; Roberta Seragliab; Pietro Traldi,b (2009)
A fast screening MALDI method for the detection of cocaine and its metabolites in hair
in Journal of mass spectrometry (Print); John Wiley & Sons, Ltd., Chichester (Regno Unito)
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Susanna Vogliardi,a; Donata Favretto,a; Giampietro Frison,a; Santo Davide Ferrara,a; Roberta Seragliab; Pietro Traldi,b (literal)

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1-4 : Forensic Toxicology and Antidoping Unit, University Hospital of Padova, viaFalloppio 50, I-35121 Padova, Italy / 5-6 : CNR, ISTM, Corso Stati Uniti 4, I35100 Padova, Italy (literal)

Titolo

A fast screening MALDI method for the detection of cocaine and its metabolites in hair (literal)

Abstract

Matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry was used for the rapid detection of cocaine, benzoylecgonine and cocaethylene in hair. Different MALDI sample preparation procedures have been tested and the employment of a multi-layer 'graphite-sample-electrosprayed ?-cyano-4-hydroxycinnamic acid (HCCA)' yielded the best results for standard solutions of the target analytes. The same approach was subsequently applied to hair samples that were known to contain cocaine, benzoylecgonine and cocaethylene, as determined by a classical GC-MS method. It was however necessary to extracthair samples byincubatingtheminmethanol/trifluoroacetic acid for a short time (15min) at 45 oC; 1 ?lof the obtained supernatantwas deposed on a metal surface treated with graphite, andHCCAwas electrosprayed on it. This procedure successfully suppressed matrix peaks and was effective in detecting all the target analytes as their protonated species. The results obtained give further confirmation of the effectiveness of the MALDI for detecting drugs and their metabolites in complex biological matrices. The method can be useful as a fast screening procedure to detect the presence of cocaine and metabolites in hair samples. (literal)

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