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Low molecular weight proteins in urines from healthy subjects as well as diabetic, nephropathic and diabetic-nephropathic patients: a MALDI study (Articolo in rivista)

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Low molecular weight proteins in urines from healthy subjects as well as diabetic, nephropathic and diabetic-nephropathic patients: a MALDI study (Articolo in rivista) (literal)

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Annunziata Lapolla; Roberta Seraglia; Laura Molin; KatherineWilliams; Chiara Cosma; Rachele Reitano; Annalisa Sechi; Eugenio Ragazzi; Pietro Traldi (2009)
Low molecular weight proteins in urines from healthy subjects as well as diabetic, nephropathic and diabetic-nephropathic patients: a MALDI study
in Journal of mass spectrometry (Print); John Wiley & Sons, Ltd., Chichester (Regno Unito)
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Annunziata Lapolla; Roberta Seraglia; Laura Molin; KatherineWilliams; Chiara Cosma; Rachele Reitano; Annalisa Sechi; Eugenio Ragazzi; Pietro Traldi (literal)

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1,5,6,7 : Department of ClinicalandSurgical Sciences,PadovaUniversity,ViaGiustiniani 2, I35100 Padova, Italy / 2,9 : CNR-ISTM, Corso Stati Uniti 4, I35100 Padova, Italy / 3 : ISB-Ion Source and Biotechnologies, Via Fantoli 16/15, I20138 Milano, Italy / 4 : Applied Biosystems, 850 Lincol Centre Drive, Foster City, CA94404, USA / 8 : Department of Pharmacology and Anesthesiology, Padova University, Largo Meneghetti 2, I35131 Padova, Italy (literal)

Titolo

Low molecular weight proteins in urines from healthy subjects as well as diabetic, nephropathic and diabetic-nephropathic patients: a MALDI study (literal)

Abstract

Urine samples from healthy subjects as well as diabetic, nephropathic and diabetic-nephropathic patients were analyzed by matrix assisted laser desorption/ionization (MALDI) mass spectrometry in order to establish evidence of some possible differencesinthepeptideprofile related tothepathological states. Multivariate analysissuggestedthepossibilityofadistinction among the considered groups of patients. Some differences have been found, in particular, in the relative abundances of three ions at m/z 1912, 1219 and 2049. For these reasons, further investigation was carried out by MALDI/TOF/TOF to determine the sequence of these peptides and, consequently, to individuate their possible origin. By this approach, the peptide at m/z 1912 was found to originate from uromodulin, and its lower expression in the case of nephropathy can be well related to the pathological condition. Ions at m/z 2049 and 1219 originate from the collagen ?-1(I) chain precursor and from the collagen ?-5 (IV) chain precursor, respectively, and, also in this case, their different expressions can be related to the pathologies under investigation. The obtained data seem to indicate that urine is an interesting biological fluid to investigate on the peptide profile and to obtain, consequently, information on the dismetabolism activated by specific pathologies. (literal)

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