Quantification of Soyasaponins I and beta g in Italian Lentil Seeds by Solid-Phase Extraction (SPE) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Quantification of Soyasaponins I and beta g in Italian Lentil Seeds by Solid-Phase Extraction (SPE) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• Sagratini, G.; Zuo, Y.T.; Caprioli, G.; Cristalli, G.; Giardina, D.; Maggi, F.; Molin, L.; Ricciutelli, M.; Traldi, P.; Vittori, S. (2009)
  Quantification of Soyasaponins I and beta g in Italian Lentil Seeds by Solid-Phase Extraction (SPE) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)
  in Journal of agricultural and food chemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Sagratini, G.; Zuo, Y.T.; Caprioli, G.; Cristalli, G.; Giardina, D.; Maggi, F.; Molin, L.; Ricciutelli, M.; Traldi, P.; Vittori, S. (literal)

Pagina inizio

• 11226 (literal)

Pagina fine

• 11233 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 57 (literal)

Rivista

• Journal of agricultural and food chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• DOI: 10.1021/jf901707z (literal)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Quantification of Soyasaponins I and beta g in Italian Lentil Seeds by Solid-Phase Extraction (SPE) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) (literal)

Abstract

• Lentil saponins are triterpene glycosides, mainly soyasaponins I and beta g (also known as VI), with multiple health-promoting properties. This paper reports the isolation of soyasaponins I and beta g from soybeans as analytical standards and the development of a new analytical procedure for quantification of their content in various cultivars of Italian lentils, by SPE-HPLC-MS. Soyasaponins I and beta g were isolated from soybeans at a purity of >90% and characterized by MS/MS (ion trap) experiments. The determination of soyasaponins in lentils was performed by extraction, SPE purification, and HPLC-MS (single quadrupole) analysis; results were confirmed by MALDI-TOF experiments. Calibration curves for soyasaponin I and beta g showed correlation coefficients of 0.998 and 0.997, respectively. LOD and LOQ values were 0.02 and 0.2 mg kg(-1) for soyasaponins I and 0.1 and 1 mg kg(-1) for soyasaponin beta g. Recoveries calculated at a 100 mg kg(-1) fortification level ranged from 85 to 97%, with n = 10 and RSs of <12%. In the 32 lentil samples, contents of soyasaponin I ranged from 28 to 407 mg kg(-1), whereas that of soyasaponin beta g ranged from 110 to 1242 mg kg(-1). (literal)

Prodotto di

• Identificazione biomarcatori proteici per diabete, nefropatie e tumori mediante spettrometria di massa (PM.P06.008.001) (Modulo)
• Institute of molecular science and technologies (ISTM) (Istituto)

Autore CNR

• PIETRO TRALDI (Persona)

Incoming links:

Prodotto

• Institute of molecular science and technologies (ISTM) (Istituto)
• Identificazione biomarcatori proteici per diabete, nefropatie e tumori mediante spettrometria di massa (PM.P06.008.001) (Modulo)

Autore CNR di

• PIETRO TRALDI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of agricultural and food chemistry (Rivista)