http://www.cnr.it/ontology/cnr/individuo/prodotto/ID48112

Do collisions inside the collision cell play a relevant role in CID-LIFT experiments? (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Do collisions inside the collision cell play a relevant role in CID-LIFT experiments? (Articolo in rivista) (literal)

Anno

2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1002/jms.1151 (literal)

Alternative label

Gloriano Moneti; Simona Francese; Guido Mastrobuoni; Giuseppe Pieraccini; Roberta Seraglia; Giovanni Valitutti; Pietro Traldi (2007)
Do collisions inside the collision cell play a relevant role in CID-LIFT experiments?
in Journal of mass spectrometry (Print); John Wiley & Sons, Ltd., Chichester (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Gloriano Moneti; Simona Francese; Guido Mastrobuoni; Giuseppe Pieraccini; Roberta Seraglia; Giovanni Valitutti; Pietro Traldi (literal)

Pagina inizio

117 (literal)

Pagina fine

126 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://onlinelibrary.wiley.com/doi/10.1002/jms.1151/abstract (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

42 (literal)

Rivista

Journal of mass spectrometry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

1 (literal)

Note

Scopu (literal)
ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1-4 : CISM, viale G.Pieraccini, 6 50139 Firenze, Italy 5-7 : ISTM-CNR, C.so Stati Uniti, 4 35127 Padova, Italy (literal)

Titolo

Do collisions inside the collision cell play a relevant role in CID-LIFT experiments? (literal)

Abstract

Collision experiments are a valid approach to characterize the ionic species generated by matrix assisted laser desorption ionization (MALDI). When a time-of-flight analyzer is employed, three different approaches are available for these experiments: the postsource decay (PSD), the LIFT and the MALDITOF/ TOF. The last two are of particular interest because of the overcoming of the PSD problems related to mass calibration of the product ion spectra. Experiments performed by LIFT on linear or cyclic peptides, in presence or in absence of collision gas in the collision cell, gave evidence of an unexpected behavior: the two spectra were practically superimposable, and in the former case only a few new fragmentation channels were activated with low yield. These results mean that the selected ion exhibits a large amount of internal energy, capable of promoting fragmentation processes in the time window corresponding to the flight time between ion source and the acceleration electrode placed after the collision cell. Experiments performed by varying the plume density show that this internal energy uptake occurs in the expanding plume, throughmultiple collisions. The LIFT data have been compared with those achieved by collisions of ESI-generated [MH]+ ions of angotensin II performed under 'in-source' conditions and by triple-quadrupole experiments. The obtained results show a strong similarity among the spectra, indicating that the internal energy uptake in a MALDI source is comparable with that of 40-eV ions colliding with Ar in a triple-quadrupole instrument. Copyright ? 2006 John Wiley & Sons, Ltd. (literal)

Editore