Anti-tumor immunity induced by CDR3-based DNA vaccination in a murine B-cell lymphoma model (Articolo in rivista)

Type
Label
  • Anti-tumor immunity induced by CDR3-based DNA vaccination in a murine B-cell lymphoma model (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.bbrc.2008.03.076 (literal)
Alternative label
  • Rinaldi, M 1; Fioretti, D 1; Iurescia, S 1; Signori, E 1,2; Pierimarchi, P 1; Seripa, D 3; Tonon, G 4; Fazio, VM 2,5 (2008)
    Anti-tumor immunity induced by CDR3-based DNA vaccination in a murine B-cell lymphoma model
    in Biochemical and biophysical research communications (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Rinaldi, M 1; Fioretti, D 1; Iurescia, S 1; Signori, E 1,2; Pierimarchi, P 1; Seripa, D 3; Tonon, G 4; Fazio, VM 2,5 (literal)
Pagina inizio
  • 279 (literal)
Pagina fine
  • 284 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • KeyWords Plus: IMMUNOGLOBULIN HEAVY-CHAIN; IDIOTYPE VACCINATION; MULTIPLE-MYELOMA; SKELETAL-MUSCLE; DENDRITIC CELLS; VACCINES; IMMUNIZATION; EXPRESSION; RESPONSES; PLASMID Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA Web of Science Category: Biochemistry & Molecular Biology; Biophysics Subject Category: Biochemistry & Molecular Biology; Biophysics IDS Number: 296EM ISSN: 0006-291X (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 370 (literal)
Rivista
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  • Epub 2008 Mar 24 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. CNR, Inst Neurobiol & Mol Med, I-00133 Rome, Italy 2. CIR, Sect Mol Med & Biotechnol, I-00155 Rome, Italy 3. IRCCS Casa Sollievo Sofferenza, Dept Res, Lab Gerontol & Geriatr, I-71013 San Giovanni Rotondo, Italy 4. Consorzio 21 Polaris, Bio Ker sri, I-09010 Pula, CA, Italy 5. IRCCS Casa Sollievo Sofferenza, Dept Res, Lab Oncol, I-71013 San Giovanni Rotondo, Italy (literal)
Titolo
  • Anti-tumor immunity induced by CDR3-based DNA vaccination in a murine B-cell lymphoma model (literal)
Abstract
  • The idiotypic structure present on B-cell neoplasms is a tumor-specific antigen and an attractive target for immunotherapy. Here, the tumor protective effects recruited by CDR3-based DNA vaccines in the poorly immunogenic, highly aggressive 38C13 murine B-cell lymphoma model were evaluated. The regions belonging to the idiotypic V(H) and V(L) CDR3 sequences were chosen for the design of two synthetic mini-genes and arranged in high-level expression plasmids. Syngeneic C3H/HeN mice were immunized by intramuscular electroporation with pV(H)CDR3-IL-2 and pV(L)CDR3-IL-2 naked DNAs. This approach provided protection in about 60% of animals challenged with a 2-fold lethal dose of tumor cells, as opposed to non-survivors in control groups. Furthermore, a long-term survival was induced in these mice since they were still alive and tumor-free 4 months following tumor challenge. Analysis of the humoral immunity revealed the presence of antibodies reactive with the peptides encompassing the CDR3 sequences in the sera of vaccinated mice. Moreover, immune sera specifically reacted with the parental 38C13 tumor cells in flow cytometry assays, indicating that such immunization elicited anti-idiotypic antibodies. These findings provide a basis for exploring the use of CDR3-based DNA vaccines against B-cell lymphoma. (literal)
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