Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Alternative label

• De Girolamo A., McKeague M., Miller J.D., De Rosa M.C., Visconti A. (2011)
  Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column
  in Food chemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• De Girolamo A., McKeague M., Miller J.D., De Rosa M.C., Visconti A. (literal)

Pagina inizio

• 1378 (literal)

Pagina fine

• 1384 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 127 (literal)

Rivista

• Food chemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• ISPA-Institute of Sciences of Food Production Carleton University (literal)

Titolo

• Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column (literal)

Abstract

• A DNA aptamer with high affinity and specificity to ochratoxin A (OTA) was conjugated to a coupling gel and used as sorbent for the preparation of solid phase extraction (SPE) columns. The SPE columns packed with 300 ll oligosorbent (24 nmol DNA) showed a linear (r = 0.999) behaviour in the range of 0.4-500 ng OTA. After optimisation of the extraction step, SPE columns were used for clean-up of OTA from wheat prior to liquid chromatographic (HPLC) analysis with fluorescence detection (FLD). Average recoveries from wheat samples spiked at levels of 0.5-50 ng/g ranged from 74% to 88% (relative standard deviation <6%) with limits of detection and of quantification of 23 and 77 pg/g, respectively. The comparative HPLC/FLD analyses of 33 naturally contaminated durum wheat samples cleaned-up on both aptamer- SPE and immunoaffinity (IMA) columns showed a good correlation (r = 0.990). Aptamer-SPE columns could be re-used up to five times without any loss of performance. (literal)

Prodotto di

• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)
• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)

Autore CNR

• ANNALISA DE GIROLAMO (Unità di personale interno)
• ANGELO VISCONTI (Unità di personale interno)

Incoming links:

Autore CNR di

• ANGELO VISCONTI (Unità di personale interno)
• ANNALISA DE GIROLAMO (Unità di personale interno)

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Food chemistry (Rivista)