http://www.cnr.it/ontology/cnr/individuo/prodotto/ID46545

Characterization of genes associated with induced resistance against Penicillium expansum in apple fruit treated with quercetin. (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Characterization of genes associated with induced resistance against Penicillium expansum in apple fruit treated with quercetin. (Articolo in rivista) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.postharvbio.2009.11.010 (literal)

Alternative label

Sanzani S.M., Schena L., De Girolamo A., Ippolito A., Candelas L.G. (2010)
Characterization of genes associated with induced resistance against Penicillium expansum in apple fruit treated with quercetin.
in Postharvest biology and technology (Print); ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Sanzani S.M., Schena L., De Girolamo A., Ippolito A., Candelas L.G. (literal)

Pagina inizio

1 (literal)

Pagina fine

11 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

56 (literal)

Rivista

Postharvest biology and technology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

11 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

1 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Annalisa De Girolamo, Institute of Sciences of Food Production, CNR, Via G. Amendola 122/O, 70126 Bari, Italy Simona Marianna Sanzani, Antonio Ippolito, Department of Plant Protection and Applied Microbiology, University of Bari, Via G. Amendola 165/A, 70126 Bari, Italy Leonardo Schena Department of Management of Agricultural and Forest Systems, Mediterranean University of Reggio Calabria, 89060 Feo di Vito, Reggio Calabria, Italy Luis González-Candelas Instituto de Agroquimica y Tecnologia de Alimentos (IATA), Consejo Superior de Investigaciones Científicas, Apdo. 73, 46100 Burjassot, Valencia, Spain (literal)

Titolo

Characterization of genes associated with induced resistance against Penicillium expansum in apple fruit treated with quercetin. (literal)

Abstract

Penicillium expansum causes blue mould, a serious postharvest disease of apples, and is the main producer of the mycotoxin patulin. Since control by synthetic fungicides is less accepted by consumers, the demand for alternative means is pressing. In a recent study, the flavonoid quercetin, although scarcely effective in in vitro assays against P. expansum growth, significantly reduced blue mould rots on 'Golden Delicious' apples, suggesting an enhancement of host disease resistance. To confirm or reject this hypothesis, genes differentially expressed in quercetin-treated 'Golden Delicious' apples were identified by suppression subtractive hybridization (SSH). A pool of 88 unique gene transcripts was obtained. Several sequences revealed high similarities with different classes of pathogenesis-related proteins (RNase-like PR10 and PR8), or with proteins expressed under stress conditions. Other transcripts had high similarity to genes of unknown function or genes coding for proteins having a role in pathogen recognition and in signalling pathways. SSH data were validated by analysing the expression of 14 genes by quantitative real-time PCR (qPCR). Eleven genes proved to be up-regulated at a medium-high level in freshly harvested apples. Among these, 5 genes selected for temporal expression profiling revealed the existence of a combined effect, particularly at 24 or 48 h, between wounding and phenolic treatment. These results provide evidence that quercetin induces resistance to P. expansum in apples. by acting on the transcription level of genes involved in several distinct metabolic processes. (literal)

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