A DNA array-based assay for the characterization of microbial community in raw milk. (Articolo in rivista)

Type
Label
  • A DNA array-based assay for the characterization of microbial community in raw milk. (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.mimet.2009.05.015 (literal)
Alternative label
  • Maria Laura Giannino; Michele Aliprandi;Maria Feligini; Laura Vanoni; Milena Brasca; Fabio Fracchetti (2009)
    A DNA array-based assay for the characterization of microbial community in raw milk.
    in Journal of microbiological methods
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Maria Laura Giannino; Michele Aliprandi;Maria Feligini; Laura Vanoni; Milena Brasca; Fabio Fracchetti (literal)
Pagina inizio
  • 181 (literal)
Pagina fine
  • 188 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 78 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 9 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopus (literal)
  • PubMe (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto Sperimentale Italiano Lazzaro Spallanzani, via Einstein, località Cascina Codazza, 26900 Lodi, Italy Istituto di Scienze delle Produzioni Alimentari (ISPA-CNR), Via Celoria, 2, 20133 Milan, Italy Laboratorio di Microbiologia, Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, Strada Le Grazie 15, Cà Vignal 2, 37134 Verona, Italy (literal)
Titolo
  • A DNA array-based assay for the characterization of microbial community in raw milk. (literal)
Abstract
  • An oligonucleotide array based on PCR method containing a combination of probes for taxonomic markers and species-specific virulence genes was developed for the simultaneous identification of 14 Lactic Acid Bacteria (LAB) and food-borne pathogenic bacteria in raw milk. The hypervariable regions V3 and V6 of 16S, together with a variable region of 23S rRNA genes and several genes specific for virulence factors were selected. Universal primers and multiplex PCR were used for the rapid differentiation of bacterial species and low concentration of specific pathogenic and spoilage bacteria were detected in milk. The dominant species such as Streptococcus thermophilus, Enterococcus faecalis, Lactococcus lactis, andLeuconostoc lactis were identified by indirect-labelling reactions based upon incorporation of amino-allyl dUTPs. The results regarding food-borne pathogens detection showed highly specific hybridisation patterns with the genomic DNA from Campylobacter jejuni, Escherichia coli, Salmonella thyphimurium, Listeria monocytogenes and Yersinia enterocolitica. A clear differentiation of dominant species present in a complex microbial community such as raw milk was achieved by the application of short oligonucleotide probes which discriminate sequences differing by few nucleotides. (literal)
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