Evaluation of intra-specific diversities in Oenococcus oeni through analysis of genomic and expressed DNA. (Articolo in rivista)

Type
Label
  • Evaluation of intra-specific diversities in Oenococcus oeni through analysis of genomic and expressed DNA. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Lechiancole T., Blaiotta G., Messina D., Fusco V., Villani F., Salzano G. (2006)
    Evaluation of intra-specific diversities in Oenococcus oeni through analysis of genomic and expressed DNA.
    in Systematic and applied microbiology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lechiancole T., Blaiotta G., Messina D., Fusco V., Villani F., Salzano G. (literal)
Pagina inizio
  • 375 (literal)
Pagina fine
  • 381 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 29 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • PubMed (literal)
  • Scopus (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Università degli Studi di Napoli, Federico II, Dipartimento di Scienza degli Alimenti (literal)
Titolo
  • Evaluation of intra-specific diversities in Oenococcus oeni through analysis of genomic and expressed DNA. (literal)
Abstract
  • malolactic fermentation (MLF). Several studies have demonstrated that O. oeni is a quite homogeneous species and strains are difficult to differentiate especially when isolates from the same region are analyzed. In this study, the molecular biodiversity of O. oeni isolated from wines of the same region (Aglianico produced in Basilicata Region, Southern Italy) was evaluated with the aim of designing a molecular approach for discrimination and characterization of the isolates at the strain level. Three molecular techniques were applied: random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), restriction endonucleases analysis-pulsed field gel electrophoresis (REA-PFGE) and differential display PCR (DD-PCR). The results obtained by RAPD-PCR confirmed the difficulty in differentiating isolates. By means of REA-PFGE a higher polymorphism, often related to the origin (winery) of strains, was revealed. However, on analyzing strains isolated from the same winery, only in some cases was more than one REA-PFGE pattern obtained. By analyzing dendrograms constructed on the basis of DD-PCR profiles differentiation of strains isolated from the same winery, in some cases, could be accomplished. The reliability of the DD-PCR in the differentiation of closely related strains suggests that this method could represent an alternative and/or additional tool to other molecular methods, such as REA-PFGE, for fine characterization of oenococcal strains. (literal)
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