Use of itaconic acid-based polymers for solid-phase extraction of deoxynivalenol and application to pasta analysis. (Articolo in rivista)

Type
Label
  • Use of itaconic acid-based polymers for solid-phase extraction of deoxynivalenol and application to pasta analysis. (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.aca.2008.01.004 (literal)
Alternative label
  • Pascale M.; De Girolamo A.; Visconti A.; Magan N.; Chianella I.; Piletska E.V.; Piletsky S.A. (2008)
    Use of itaconic acid-based polymers for solid-phase extraction of deoxynivalenol and application to pasta analysis.
    in Analytica chimica acta (Print); Elsevier B.V., Amsterdam (Belgio)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pascale M.; De Girolamo A.; Visconti A.; Magan N.; Chianella I.; Piletska E.V.; Piletsky S.A. (literal)
Pagina inizio
  • 131 (literal)
Pagina fine
  • 138 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 609 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Michelangelo Pascale, Annalisa De Girolamo, Angelo Visconti - Istituto di Scienze delle Produzioni Alimentari, Consiglio Nazionale delle Ricerche, Via Amendola 122/O, 70126 Bari, Italy; 2 Cranfield Health, Cranfield University, Silsoe, Bedforshire MK45 4DT, UK (literal)
Titolo
  • Use of itaconic acid-based polymers for solid-phase extraction of deoxynivalenol and application to pasta analysis. (literal)
Abstract
  • Molecular modelling and computational design were used to identify itaconic acid (IA) as a functional monomer with high affinity towards deoxynivalenol (DON), a Fusariumtoxin frequently occurring in cereals. IA-based polymers were photochemically synthesised in dimethyl formamide (porogen) using ethylenglycol dimethacrylate as cross-linker and 1,1'-azo-bis(cyclohexane carbonitrile) as initiator, and the relevant binding interactions with DON in solvents with different polarity were investigated. The performances of the non-imprinted IA-based polymer (blank polymer, BP) and the corresponding molecularly imprinted polymer (MIP) were compared using DON as a template. Both BP and MIP were able to bind about 90% DON either in toluene, water or water containing 5% polyethylene glycol. Non-imprinted polymers with different molar ratios of IA to cross-linker were evaluated as adsorbents for solid-phase extraction (SPE) clean-up and pre-concentration of DON from wheat and pasta samples prior to HPLC analysis. Samples were extracted with PBS/0.1 M EDTA solution and cleaned up through a cartridge containing blank IA-based polymer. The column was washed with PBS (pH 9.2) and the toxin was eluted with methanol and quantified by reversed-phase HPLC with UV detector (lambda = 220 nm), using methanol:water: acetic acid (15:85:0.1, v/v/v) as the mobile phase. Effective removal of matrix interferences was observed only for pasta with DON recoveries higher than 70% (RSD < 7%, n = 3) at levels close to or higher than EU regulatory limit. (literal)
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