Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.chroma.2005.04.009 (literal)

Alternative label

• Visconti A.; Lattanzio V.M.T.; Pascale M.; Haidukowski M. (2005)
  Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection.
  in Journal of chromatography (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Visconti A.; Lattanzio V.M.T.; Pascale M.; Haidukowski M. (literal)

Pagina inizio

• 151 (literal)

Pagina fine

• 158 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1075 (literal)

Rivista

• Journal of chromatography (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Il lavoro riporta lo sviluppo di un metodo cromatografico per la determinazione simultanea delle tossine T-2 e HT-2 in frumento, mais e orzo. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Sciences of Food Production, National Research Council, Via G. Amendola 122/O, 70126, Bari, Italy. (literal)

Titolo

• Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection. (literal)

Abstract

• A sensitive, precise and accurate method has been developed for the simultaneous determination of T-2 and HT-2 toxins in cereal grains at ppb levels using high-performance liquid chromatography (HPLC) with fluorescence detection and 1-antroylnitrile (1-AN) as labelling reagent after immunoaffinity clean-up. Cereal samples were extracted with methanol/water (90:10, v/v), and the extracts were cleaned-up through commercially available immunoaffinity columns containing monoclonal anti-T-2 antibodies (T-2 testTM HPLC, Vicam). T-2 and HT-2 toxins were quantified by reversed-phase HPLC with fluorometric detection (excitation wavelength 381 nm, emission wavelength 470 nm) after derivatization with 1-AN. The monoclonal antibody showed 100% cross-reactivity with both T-2 and HT-2 toxin, and the immunoaffinity column clean-up was effective up to 1.4 µg of both toxins. The method was successfully applied to the analysis of T-2 and HT-2 toxins in wheat, maize and barley. Recoveries from spiked samples with toxin levels from 25 to 500 µg/kg ranged from 70% to 100%, with relative standard deviation generally lower than 8%. The limit of detection of the method was 5 µg/kg for T-2 toxin and 3 µg/kg for HT-2 toxin, based on a signal-to-noise ratio 3:1. HT-2 toxin was detected in ten naturally contaminated wheat samples out of 14 samples analyzed, with toxin levels ranging from 10 to 71 µg/kg; three of them contained also T-2 toxin up to 12 µg/kg. (literal)

Prodotto di

• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)
• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)

Autore CNR

• EDITH MIRIAM HAIDUKOWSKI (Unità di personale interno)
• ANGELO VISCONTI (Unità di personale interno)
• MICHELANGELO PASCALE (Unità di personale interno)
• VERONICA MARIA TERESA LATTANZIO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ANGELO VISCONTI (Unità di personale interno)
• EDITH MIRIAM HAIDUKOWSKI (Unità di personale interno)
• MICHELANGELO PASCALE (Unità di personale interno)
• VERONICA MARIA TERESA LATTANZIO (Unità di personale interno)

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of chromatography (Rivista)

Insieme di parole chiave di

• Parole chiave di "Analysis of T-2 and HT-2 toxins in cereal grains by immunoaffinity clean-up and liquid chromatography with fluorescence detection." (Insieme di parole chiave)