MALDI-TOF mass spectrometry of a combinatorial peptide library: effect of matrix composition on signal suppression (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• MALDI-TOF mass spectrometry of a combinatorial peptide library: effect of matrix composition on signal suppression (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/jms.937 (literal)

Alternative label

• Schlosser Gitta, Pocsfalvi Gabriella, Huszar Emoke, Malorni Antonio, Hudecz Ferenc (2005)
  MALDI-TOF mass spectrometry of a combinatorial peptide library: effect of matrix composition on signal suppression
  in Journal of mass spectrometry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Schlosser Gitta, Pocsfalvi Gabriella, Huszar Emoke, Malorni Antonio, Hudecz Ferenc (literal)

Pagina inizio

• 1590 (literal)

Pagina fine

• 1594 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• 23rd Informal Meeting on Mass Spectrometry (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 19 (literal)

Rivista

• Journal of mass spectrometry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 12 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518 Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, Avellino, 83100, Italy Department of Organic Chemistry, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518 (literal)

Titolo

• MALDI-TOF mass spectrometry of a combinatorial peptide library: effect of matrix composition on signal suppression (literal)

Abstract

• The effect of matrix composition on signal suppression caused by a dominant compound under MALDI ionization was studied using the combinatorial TQTXT pentapeptide library as a model system. The peptide library is composed of 19 components with all proteinogenic amino acids except cysteine in position X. From these compounds, only the Arg peptide (TQTRT) was detected with sufficient intensity in the MALDI-TOF mass spectrum under typical MALDI conditions (CCA matrix). The analysis of a set of compounds utilized as different matrix components, additives and a cationizing agent revealed that the composition of the matrix is a critical point in signal suppression. Highly improved ion yields were achieved by using a CCA/DHB mixture as a matrix. The addition of K(+) as a cationizing agent to the CCA matrix resulted in MALDI-TOF mass spectra with relative ion intensities very similar to those obtained by electrospray ionization. (literal)

Prodotto di

• Metodologie di Spettrometria di Massa, Proteomica, Metabolomica e Bioinformatica nelle Scienze dell'Alimentazione (AG.P05.003.001) (Modulo)
• Institute of food sciences (ISA) (Istituto)

Autore CNR

• ANTONIO MALORNI (Unità di personale interno)
• GABRIELLA KATALIN POCSFALVI (Persona)

Incoming links:

Prodotto

• Institute of food sciences (ISA) (Istituto)
• Metodologie di Spettrometria di Massa, Proteomica, Metabolomica e Bioinformatica nelle Scienze dell'Alimentazione (AG.P05.003.001) (Modulo)

Autore CNR di

• ANTONIO MALORNI (Unità di personale interno)
• GABRIELLA KATALIN POCSFALVI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of mass spectrometry (Rivista)