http://www.cnr.it/ontology/cnr/individuo/prodotto/ID45606
Mutations of the CK2 phosphorylation site of Sic1 affect cell size and S-Cdk kinase activity in Saccharomyces cerevisiae. (Articolo in rivista)
- Type
- Label
- Mutations of the CK2 phosphorylation site of Sic1 affect cell size and S-Cdk kinase activity in Saccharomyces cerevisiae. (Articolo in rivista) (literal)
- Anno
- 2004-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1046/j.1365-2958.2003.03836.x (literal)
- Alternative label
Coccetti P, Rossi RL, Sternieri F, Porro D, Russo GL, Di Fonzo A, Magni F, Vanoni M, Alberghina L. (2004)
Mutations of the CK2 phosphorylation site of Sic1 affect cell size and S-Cdk kinase activity in Saccharomyces cerevisiae.
in Molecular microbiology (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Coccetti P, Rossi RL, Sternieri F, Porro D, Russo GL, Di Fonzo A, Magni F, Vanoni M, Alberghina L. (literal)
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- L'articolo è open access su:
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2003.03836.x/pdf (literal)
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- Rivista
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- Dipartimento di Biotecnologie e Bioscienze, Università degli Studi Milano-Bicocca
Dipartimento di Medicina Sperimentale, Ambientale e Biotecnologie Mediche, Università degli Studi Milano Bicocca
Istituto di Scienze dell'Alimentazione, CNR, Avellino (literal)
- Titolo
- Mutations of the CK2 phosphorylation site of Sic1 affect cell size and S-Cdk kinase activity in Saccharomyces cerevisiae. (literal)
- Abstract
- By sequence analysis we found an amino acid stretch
centred on Serine
201
matching a stringent CK2 consensus
site within the C-terminal, inhibitory domain
of Sic1. Here we show by direct mass spectrometry
analysis that Sic1, but not a mutant protein whose
CK2 phospho-acceptor site has been mutated to alanine,
Sic1
S201A
, is actually phosphorylated
in vitro
by
CK2 on Serine 201. Mutation of Serine 201 alters the
coordination between growth and cell cycle progression.
A significant increase of average protein content
and of the average protein content at the onset of DNA
synthesis is observed for exponentially growing cells
harbouring the Sic1
S201A
protein. A strong reduction of
the same parameters is observed in cells harbouring
Sic1
S201E
. The deregulated coordination between cell
size and cell cycle is also apparent at the level of SCdk
activity. (literal)
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