Nerve Growth Factor-dependent survival of CESS B cell line is mediated by increased expression and decreased degradation of MAPK Phosphatase 1 (Articolo in rivista)

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  • Nerve Growth Factor-dependent survival of CESS B cell line is mediated by increased expression and decreased degradation of MAPK Phosphatase 1 (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1074/jbc.M305356200 (literal)
Alternative label
  • Rosini P.1; De Chiara G.2; Bonini P.1; Lucibello M.2,3, Marcocci M.E.2; Garaci E.2; Cozzolino 1,2,3; Torcia M.G.1; (2004)
    Nerve Growth Factor-dependent survival of CESS B cell line is mediated by increased expression and decreased degradation of MAPK Phosphatase 1
    in Journal of biological chemistry (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Rosini P.1; De Chiara G.2; Bonini P.1; Lucibello M.2,3, Marcocci M.E.2; Garaci E.2; Cozzolino 1,2,3; Torcia M.G.1; (literal)
Pagina inizio
  • 14016 (literal)
Pagina fine
  • 14023 (literal)
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  • Impact Factor 6.482 (literal)
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  • 279 (literal)
Rivista
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  • 14 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1= Department of Clinical Physiopathology, University of Florence, Florence, Italy; 2= Department of Experimental Medicine, University of Rome \"Tor Vergata\", Via di Tor Vergata 135, I-00133 Rome, Italy 3= Institute of Neurobiology and Molecular Medicine, National Research Council, Via del Fosso del Cavaliere 100, I-00133 Rome, Italy; (literal)
Titolo
  • Nerve Growth Factor-dependent survival of CESS B cell line is mediated by increased expression and decreased degradation of MAPK Phosphatase 1 (literal)
Abstract
  • The sIgG(+) lymphoblastoid B cell line CESS spontaneously produces a high amount of nerve growth factor (NGF) and expresses both high affinity (p140(Trk-A)) and low affinity (p75(NTR)) NGF receptors. Autocrine production of NGF maintains the survival of CESS cells through the continuous deactivation of p38 MAPK, an enzyme able to induce Bcl-2 phosphorylation and subsequent cytochrome c release and caspase activation. In this paper, we show that NGF induces transcriptional activation and synthesis of MAPK phosphatase 1 (MKP-1), a dual specificity phosphatase that dephosphorylates p38 MAPK, thus preventing Bcl-2 phosphorylation. Furthermore, NGF increases MKP-1 protein stability by preventing its degradation through the proteasome pathway. Following NGF stimulation, MKP-1 protein mainly localizes on mitochondria, suggesting an interaction with p38 MAPK in this compartment. Incubation of CESS cells with MKP-1-specific antisense oligonucleotides induces cell death, which was not prevented by exogenous NGF. By contrast, overexpression of native MKP-1, but not of its catalytically impaired form, inhibits apoptosis induced by NGF neutralization in CESS cells. Thus, the molecular mechanisms underlying the survival function of NGF in CESS B cell line predominantly consist in maintaining elevated levels of MKP-1 protein, which controls p38 MAPK activation. (literal)
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